DNA/ RNA Synthesizer
Make
K & A lab GMBH
Model
H6 DNA/ RNA Synthesizer
Facility Status
Working
Date of Installation
Facility Management Division
Institute Central Research Facilities (ICRF)

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Category

  • Synthesis ยป Biochemical Synthesis

Booking Details

Booking available for
Internal and External Both
Available Equipment/ Mode of use
solid phase oligonucleotide synthesis of modified building blocks of DNA and RNA.

Facility Management Team and Location

Faculty In Charge
Prof. Pradeepkumar P. I
Prof. Nandita Madhavan
Facility Manager
Mr. Amol Hanumante
Facility Management Members
Prof. Pradeepkumar P. I
Prof. Nandita Madhavan
Prof Ruchi Anand
Prof. Venkateswarlu Yarlagadda
Prof Kiran Kondabagil
Department
Chemistry
Lab Email ID
amol_ah@iitb.ac.in
Facility Location
Room No 212A, Chemistry Department Facility lab IV, First floor, Chemistry Building, IIT Bombay
Lab Phone No
022-25764159

Facility Features, Working Principle and Specifications

Facility Description

Facility Description

This automated synthesizer is used for solid phase oligonucleotide synthesis containing particular sequence of nucleotides. 

Features Working Principle

Specification / Features: 

  • Scale of synthesis: 200nmol / 1 umol/ 10umol 
  • Number of columns: 6 columns 
  • Number of amidite positions: upto 12 amidite positions 
  • Number of reagent positions: 6 positions 

 

 

Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures

Instructions for Registration

User must contact the Technical operator before booking slot and discuss the experimental parameters

 

Instruction for Sample Preparation
  • Chemicals/Reagents/ solvents are to be supplied by end user. 
  • Argon Charges are extra.  (Minimum charges are Rs 250 per day )are to be supplied by end user.  
  • If end users do not supply chemicals, reagents/solvents, in addition to above basic charges, there will be additional charges as per the nucleotide sequence. The end-user will be provided a quotation for the same.

     

User Instructions and Precautionary Measures
  • The facility caters to custom incorporation of modified building blocks of DNA and RNA. 
  • Internal User must get trained before using this Instrument.

     

Charges for Analytical Services in Different Categories

Usage Charges

Charges in INR:

Type of Analysis

IITB users

Academic Institutions

National R&D labs

Industries / Non Govt. Agencies

Charges per day

 

500

1000*

2500*

10000*

All charges in INR. GST extra for external samples.

Additional Argon gas charges: Rs 250 per day (Minimum charges are Rs 250)

These are only Instrument/ recording charges. Chemicals/Reagents/ solvents are to be supplied by end user. 

Applications

Sample Details

Chemical allowed

Yes

Allowed Substrate

No

Gases allowed

No

Substrate Dimension

No

Target dimension

No

Contamination remarks

No

Precursors/ Targets allowed

No

SOP, Lab Policies and Other Details

Publications