External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
.
Category
- Synthesis » Biochemical Synthesis
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
This automated synthesizer is used for solid phase oligonucleotide synthesis containing particular sequence of nucleotides.
Specification / Features:
- Scale of synthesis: 200nmol / 1 umol/ 10umol
- Number of columns: 6 columns
- Number of amidite positions: upto 12 amidite positions
- Number of reagent positions: 6 positions
Sample Preparation, User Instructions and Precautionary Measures
- Chemicals/Reagents/ solvents are to be supplied by end user.
- Argon Charges are extra. (Minimum charges are Rs 250 per day )are to be supplied by end user.
If end users do not supply chemicals, reagents/solvents, in addition to above basic charges, there will be additional charges as per the nucleotide sequence. The end-user will be provided a quotation for the same.
- The facility caters to custom incorporation of modified building blocks of DNA and RNA.
Internal User must get trained before using this Instrument.
Charges for Analytical Services in Different Categories
Charges in INR:
| IITB (TAs) | IITB Students | IITB-Monash Students | Academic Institutes | National Labs | Sine (letter from SINE reqd.) | Research Park (MSME) (letter from RP reqd.) | Research Park (Big Industry partners) (letter from RP reqd.) | and MSME not associated with RP (appropriate certificate required) | Industries | |
| Charges | X/2 | X | X | 2X | 5X | 5X | 5X | 7.5X | 7.5X | 10X |
| DNA/ RNA Synthesizer (BIOSYNTH-02) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| Peptide Synthesizer (BIOSYNTH-03) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| Nitrogen charges for Peptide synthesizer per day additional | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 |
| HPLC(BIOSYNTH-04)per hour including pre and post washtime | 125 | 250 | 250 | 500 | 1250 | 1250 | 1250 | 1875 | 1875 | 2500 |
| Freeze Dryer (BIOSYNTH-05) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| GST | NO GST | NO GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST |
All charges in INR. GST extra for external samples.
Additional Argon gas charges: Rs 250 per day (Minimum charges are Rs 250)
These are only Instrument/ recording charges. Chemicals/Reagents/ solvents are to be supplied by end user.
Applications
Sample Details
Yes
No
No
No
No
No
No