DNA/ RNA Synthesizer

External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”

Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.

Make
K & A lab GMBH
Model
H6 DNA/ RNA Synthesizer
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)-IoE Funded

.

Category

  • Synthesis » Biochemical Synthesis

Booking Details

Booking available for
Internal and External Both
Available Equipment/ Mode of use
solid phase oligonucleotide synthesis of modified building blocks of DNA and RNA.

Facility Management Team and Location

Facility In Charge
Prof. Pradeepkumar P. I
Prof. Nandita Madhavan
Facility Manager
Mr. Amol Hanumante
Facility Management Members
Prof. Pradeepkumar P. I
Prof. Nandita Madhavan
Prof Ruchi Anand
Prof. Venkateswarlu Yarlagadda
Prof Kiran Kondabagil
Department
Chemistry
Lab Email ID
amol_ah@iitb.ac.in
Facility Location
Room No 212A, Chemistry Department Facility lab IV, First floor, Chemistry Building, IIT Bombay
Lab Phone No
022-25764159

Facility Features, Working Principle and Specifications

Facility Description

Facility Description

This automated synthesizer is used for solid phase oligonucleotide synthesis containing particular sequence of nucleotides. 

Features Working Principle

Specification / Features: 

  • Scale of synthesis: 200nmol / 1 umol/ 10umol 
  • Number of columns: 6 columns 
  • Number of amidite positions: upto 12 amidite positions 
  • Number of reagent positions: 6 positions 

 

 

Sample Preparation, User Instructions and Precautionary Measures

Instruction for Sample Preparation
  • Chemicals/Reagents/ solvents are to be supplied by end user. 
  • Argon Charges are extra.  (Minimum charges are Rs 250 per day )are to be supplied by end user.  
  • If end users do not supply chemicals, reagents/solvents, in addition to above basic charges, there will be additional charges as per the nucleotide sequence. The end-user will be provided a quotation for the same.

     

User Instructions and Precautionary Measures
  • The facility caters to custom incorporation of modified building blocks of DNA and RNA. 
  • Internal User must get trained before using this Instrument.

     

Charges for Analytical Services in Different Categories

Usage Charges

Charges in INR:

Type of Analysis

IITB users

Academic Institutions

National R&D labs

Industries / Non Govt. Agencies

Charges per day

 

500

1000*

2500*

10000*

All charges in INR. GST extra for external samples.

Additional Argon gas charges: Rs 250 per day (Minimum charges are Rs 250)

These are only Instrument/ recording charges. Chemicals/Reagents/ solvents are to be supplied by end user. 

Applications

Sample Details

Chemical allowed

Yes

Allowed Substrate

No

Gases allowed

No

Substrate Dimension

No

Target dimension

No

Contamination remarks

No

Precursors/ Targets allowed

No

SOP, Lab Policies and Other Details

Publications