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Category
- Spectroscopy and Spectrometry » Optical Spectroscopy
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
Versatile Detection Options – The system features a high performance optical system that combines low stray light with high throughput. A modular approach allows selection of the appropriate light sources and detectors according to the work being done. Multi-wavelength absorbance analysis from a single shot using the KinetaScan CCD detector can be especially useful for small quantities of samples. Fluorescence polarisation and anisotropy measurements are fully software controlled, whilst conductivity allows the study of fast conductimetric reactions.
Stability, Sensitivity And Flexibility – Reference channel optics offer outstanding stability and immunity from interference to allow even smaller signal changes to be followed and over extended time scales without the effects of drift. Ease of use and intuitive operation are the product of Kinetic Studio, our Windows-based software. This features easy to follow set up routines and auto calibration.
Operation over a wide temperature range is enhanced by the use of the low temperature option, allowing work down to -90°C. Or down to -20°C by using the innovative T-Pod option.
Innovative Design – The unique flow circuit design ensures that the minimal amount of solution is used for each experiment. This is particularly important if you are using volume limiting samples (e.g. mutant enzymes or other proteins) or equally novel materials. A robust ceramic stop/waste valve gives extended life. All components have been carefully selected for compatibility and reliability.
The robust nature and careful design of the flow circuit has led to it being an instrument of choice for users wishing to carry out their experiments under anaerobic conditions – the containment of reagents under low oxygen (anaerobic) conditions is noteworthy. For some users, the choice is to use a glove box – we have expertise in adapting instruments for use in glove boxes.
Programmable Multi-Shot Capability – Bringing added versatility for multi-wavelength analysis for both absorbance and fluorescence, even into the deep UV part of the spectrum. The KinetAsyst system is fully programmable for number of shots and wavelength, with the SF-61DX2 instrument adding age time to the list of user-controlled variables.
Kinetic Studio Speeds Data Processing – Its modern Windows interface allows Kinetic Studio to provide a host of data handling and visualisation facilities. An easy export utility brings compatibility with other data processing packages such as ReactLab, SPECFIT/32, ORIGIN, KaleidaGraph and THE SCIENTIST.
Stopped-flow is a technique that rapidly mixes two or more solutions to study fast reactions ( ms timescale), using a high-efficiency mixer, and then observing the reaction's progress in real-time through methods like absorbance or fluorescence spectroscopy
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
Internal Users
Users within IIT Bombay can apply by emailing the facility in charges after training is completed (for now).
Please click on this link to see the flow chart of the full procedure: @Link to Flow Chart
The online registration form should be completely filled out by the applicant. For any query contact the facility manager.
After getting approval from the student’s PI along with the committing charges, an appointment will be given as per queue, and will be informed by email/phone.
At least 24 hours prior information should be given to the stopped-flow lab if users want to cancel/postpone the slot.
External Users
1. External users can come personally or send the filled in registration form along with the Demand draft and a letter from the Guide / Head on the Institute Original Letter Head stating that the analysis is for research purpose, to qualify for academic concession. The letter should be addressed to “Prof. Shobhna Kapoor and Prof. Ishita Sengupta, Facility-in-charges, Stopped-flow Spectrometer, Department of Chemistry, IIT Bombay, Powai, Mumbai – 400076.”
2.Operators will have to undergo training and demonstrate independence through a test before given usership.
3. Samples can be brought along on the date of your appointment.
Samples have to be soluble, high concentration, and devoid of solid particles. buffers should not be viscous, filtered and degassed
Samples have to be soluble, high concentration, and devoid of solid particles. buffers should not be viscous, filtered and degassed. Users have to undergo training and pass a written and hands-on test before proceeding to use the instrument. The instrument has to be returned to the original configuration if settings are changed. Nitrogen gas and water bath needs to be refilled at regular intervals.
Charges for Analytical Services in Different Categories
| Internal Users | 1. Rs. 250/day (Rs. 100/day if booking is for a stretch of 5 days or more) |
| External Users (Academic) | 1. Rs. 500/day |
| External Users (National R&D Labs) | 1. Rs. 1250/day |
External Users (Industry & Non Govt. Agency) | 1. Rs. 2500/day |
Applications
fast reactions in the 2 ms timescale slower (binding, folding, scattering assays etc)
Sample Details
non corrosive
biomolecules, small molecules
none
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