Spectrofluorometer

External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”

Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.

Make
Horiba
Model
FQ5310022306A00168
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)-IoE Funded

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Category

  • Spectroscopy and Spectrometry » Optical Spectroscopy

Booking Details

Booking available for
Internal and External Both
Available Equipment/ Mode of use
Quantum yield and Phosphorescence for solid and liquids

Facility Management Team and Location

Facility In Charge
Professor Arindam Chowdhury
Facility Manager
Mr Amol Hanumante
Facility Operator
Mr Amol Hanumante
Facility Management Members
Prof. Arindam Chowdhury (Chemistry Department)
Prof. M. Ravikanth (Chemistry Department)
Prof. Shobhna Kapoor (Chemistry Department)
Prof. M. Aslam (Physics Department)
Prof. Chidambar Kulkarni (Chemistry Department)
Department
Chemistry
Lab Email ID
amol_ah@iitb.ac.in
Facility Location
Room No 360, Chemistry Department Facility lab II, 2nd floor, Chemistry Building, IIT Bombay
Lab Phone No
022-25764159

Facility Features, Working Principle and Specifications

Facility Description

Facility Description

This Spectrophotometer offers the ultimate sensitivity in fluorescence or emission profile of the complex under investigation. It is controlled by Felix software for steady state and lifetime experiments. It has wavelength range from UV to NIR and used for NIR steady state and phosphorescence lifetime detection. 

 

 

Features Working Principle

Features/ Accessories: 

  • Source: 450 W Xenon lamp
  • Pulsed Source for Lifetime study
  • Felix FL software
  • Automated polarizers located at entrance and exit of sample compartment
  • Double Excitation 350nm Czerny-Turner coma-corrected monochromators
  • Single Emission 350nm Czerny-turner coma-corrected monochromator
  • Integrating Sphere for direct PLQY sample measurement 
  • Multimode PMT detector 
  • Liquid Nitrogen cooled InGaAs detector (Range 800-1500nm) 
  • SS holder for thin film, Powdered samples

Principle: 

  1. https://www.horiba.com/ind/scientific/technologies/fluorescence-spectroscopy/what-is-fluorescence-spectroscopy/ 

2.  https://www.horiba.com/ind/scientific/technologies/fluorescence-spectroscopy/what-are-luminescence-quantum-yields/

 

Body Specification

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Sample Preparation, User Instructions and Precautionary Measures

Instruction for Sample Preparation
  • If Any reference material, solvents required are to be supplied by the user
  • User should provide solid samples in the fine powder form. Minimum required amount is approximately 1-2 gm. 
  • Liquid Samples are prepared by user in appropriate concentration required for the experiment. The required minimum amount is 2-3 ml. 
  • In case any additional Chemicals/ materials required for recording the samples, Charges would be additional.
User Instructions and Precautionary Measures

MS-DS (Material Safety Data Sheet) should be given along with samples. Samples should not be toxic or hazardous.

Charges for Analytical Services in Different Categories

Usage Charges

 

 

 IITB (TAs)IITB  StudentsIITB-Monash StudentsAcademic InstitutesNational LabsSine (letter from SINE reqd.)Research Park    (MSME)           (letter from RP reqd.)Research Park (Big Industry partners) (letter from RP reqd.)and MSME not associated with RP (appropriate certificate required)Industries
Charges in Rs per Hour or upto one hourX/2XX2X5X5X5X7.5X7.5X10X
Emission / Excitation by Fluoroscence501001002005005005007507501000
Absolute quantum Yield/ Phosphorecence at RT/ Temp dependent study for Fluoroscence or NIR region Fluoroscence100200200400100010001000150015002000
GSTNO GSTNO GST18 % GST18 % GST18 % GST18 % GST18 % GST18 % GST18 % GST18 % GST

 For external samples, in addition to above basic charges prevailing GST will be added. Note: These charges are only recording charges for Pre-made samples. It does not involve any preparation of solutions or any Data Interpretation. It does not include any cost required for solvents / other chemicals. 

Applications

  • Qualitative and Quantitative 
  • In Biotechnology 
  • life time measurement and Quantum Yield 
  • Industrial etc

Sample Details

Chemical allowed

No

Allowed Substrate

No

Gases allowed

No

Substrate Dimension

No

Target dimension

No

Contamination remarks

No

Precursors/ Targets allowed

No

SOP, Lab Policies and Other Details

Publications