AGILENT GCMS
Request form for external booking (Sample and analysis details)
External Registration Form for Semi_prep HPLC Shimadzu.docx (74.23 KB)
Request form for internal booking (Sample and analysis details)
Internal Registration Form for Semi_prep HPLC Shimadzu.docx (74.2 KB)
Make
SHIMADZU
Model
Semi-Prep CBM -20A HPLC
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)-IoE Funded

.

Category

  • Chromatography » Liquid Chromatography

Booking Details

Slot Booking Link
I-STEM booking link for External Users
Booking available for
Internal and External Both
Available Equipment/ Mode of use
Analytical and Semi prep mode

Facility Management Team and Location

Facility In Charge
Prof. Chandra M. R. Volla, Chemistry
Facility Manager
Charine Astrid Pawar
Facility Management Members
Prof. Sanjog S. Nagarkar, Chemistry
Prof. Venkateswarlu Yarlagadda
Prof. Deepti Kalsi, Chemistry
Department
Chemistry
Lab Email ID
charine@chem.iitb.ac.in
Facility Location
Room no: 370, Second Floor, Central Facility Department: Department of Chemistry
Lab Phone No
022 25764159

Facility Features, Working Principle and Specifications

Facility Description

Facility Description

Semi preparative Chromatography is carried out under low backpressures with bigger inner diameter columns and bigger particle size by passing mobile phases at high flow rates. It is used to purify and collect component of interest from all other contaminants in sample.

Features Working Principle
  • It can be used for analytical as well as Semi-Prep analysis depending upon column used and mode selected.
  • LC-20AP Pump along with FCV-200AL unit (Quaternary valve) is capable of using 4 solvents with max. flow upto 50mL/ min. 
  • Autosampler ( SIL-20AC HT) is available for Analytical mode which introduces a precise aliquot of a sample solution from a sample container to HPLC column. For Prep Mode , sample is injected manually ( max. 2mL)
  • Fraction collector module (FRC-10A): it collects fractions even when the peak elution time varies, by catching the target component according to variations in the chromatogram. 
  • PDA detector(SPD-M40): It can detects entire Wavelength range of 190 nm - 800 nm simultaneously, wavelength accuracy ± 1 nm.

Following Reverse Phase column are available with system Shim Pack GIST C18, 5um, 4.6 x 250 Shim Pack GIST C18, 5um, 10 x 250 Shim Pack GIST C18, 5um, 20 x 250

Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures

Instructions for Registration

User must contact technical operator before booking the slot and discuss about experiment parameters.

Instruction for Sample Preparation

Only solvents compatible with the column can be used. All solvents and any chemicals required to be provided by the user.

User Instructions and Precautionary Measures
  • User is expected to know the details about method and column to be used. Method suitable for column also to be provided by the end user.
  • Internal Users are supposed to bring all chemicals required for the experiment.

Charges for Analytical Services in Different Categories

Usage Charges

Charges in INR

Analysis

IITB users

Academic Institutions

National R & D Labs

Industries/   Non Govt. Agencies

Basic Charges per sample (each single recording) for analytical mode

 

Rs. 100/-

 

 

Rs. 300/-

 

Rs. 750/-

 

Rs. 1500/-

Basic Charges for Semi-prep mode run per hour of sample runtime

 

Rs. 250/-

 

 

Rs. 1000/-

 

Rs. 2000/-

 

Rs. 4000/-

 

For external samples in addition to basic charges, prevailing GST will be added.

Note: 

These are only instrument charges. Chemicals/ reagents/ solvents required for analysis will be supplied by end user.

Applications

Sample Details

Chemical allowed

Yes

Gases allowed

No

SOP, Lab Policies and Other Details

Publications