External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
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Category
- Spectroscopy and Spectrometry » Mass Spectrometry
- Chromatography » Liquid Chromatography
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
Liquid chromatography / Mass Spectroscopy (LC / MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectroscopy ,a powerful analysis & detection technique.
There are two common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI) & Atmospheric Pressure Chemical Ionization (APCI). Both are soft ionization technique.
Agilent iFunnel technology combines highly efficient electrospray ion generation and focusing of Agilent Jet Stream technology with a hexabore capillary sampling array and dual-stage ion funnel for increased ion sampling and transmission.
HPLC combined with mass spectrometer can analysed small molecule as well large molecules like proteins.
Mass spectrometry is an analytical technique that can provide both qualitative (structure) and quantitative (molecular mass or concentration) information on analyte molecules after their conversion to ions. The molecules of interest are first introduced into the ionisation source of the mass spectrometer, where they are first ionised to acquire positive or negative charges. The ions then travel through the mass analyser and arrive at different parts of the detector according to their mass/charge (m/z) ratio. After the ions make contact with the detector, useable signals are generated and recorded by a computer system. The computer displays the signals graphically as a mass spectrum showing the relative abundance of the signals according to their m/z ratio.
Specification
- Mass Range :50-3200 amu
- Mass resolving power: Greater than 45 ,000 FWHM at m/z 2,722
- Mass accuracy – MS mode: Less than 0.8 ppm RMS
- Mass accuracy – MS/MS mode: Less than 2 ppm RMS
- Mass accuracy temperature stability: Maintain 1 ppm mass accuracy (variations < 3 °C from calibration temperature)
- Sensitivity-MS mode (1 pg. Reserpine): 500: 1 RMS
- Sensitivity, MS/MS mode (1 pg. Reserpine): 1,500:1 RMS
- Direct Infusion for Mass Analysis (MS, MS /MS)
- Spectral acquisition rate, MS mode: 50 spectra/second
- Spectral acquisition rate, MS/MS mode: 30 MS/MS spectra/second
Sample Preparation, User Instructions and Precautionary Measures
Instructions for sample preparation / submission
- Sample should be pure and dry and submit in a vial along with the sample code, molecular formula or molecular weight.
- Mention the suitable solvent (preferably protonated solvent like MeOH, ACN or water)
User Instructions:
1. Expected structure or mass can be provided.
2. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent.
3. As HP-LC is available in most departments, LC of submitted samples indicating the operation should be supplied along with the sample.
4. For HPLC DAD analysis, wavelength should be given.
5. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that their sample being given. Samples should not be toxic or hazardous. Samples will not be accepted unless MS-DS.
Charges for Analytical Services in Different Categories
| Type of Analysis | without GST | with GST | |||||||||
| IITB(TA) | IITB Students | IITB Monash Students | Acadamic Institutes | National Lab | SINE(letter from sine required) | Reasearch park(MSME) letter from RP required | Reasearch park(Big Industry partners) letter from RP required | MsME not associated with RP (approriate certificate required) | Industries | ||
| X/2 | X | X | 2X | 5X | 5X | 5X | 7.5X | 7.5X | 10X | ||
| Only HRMS (with solvent and sample preparation) | 10 | 20 | 20 | 40 | 100 | 100 | 100 | 150 | 150 | 200 | Per Sample |
| Only HRMS (without solvent and sample preparation) | 50 | 100 | 100 | 200 | 500 | 500 | 500 | 750 | 750 | 1000 | Per Sample |
| HRMS + compound formula and library search (with solvent and sample preparation) | 20 | 40 | 40 | 80 | 200 | 200 | 200 | 300 | 300 | 400 | Per Sample |
| HRMS + compound formula and library search (without solvent and sample preparation) | 100 | 200 | 200 | 400 | 1000 | 1000 | 1000 | 1500 | 1500 | 2000 | Per Sample |
| LCMS (with own column, solvents & sample preparation) | 50 | 100 | 100 | 200 | 500 | 500 | 500 | 750 | 750 | 1000 | Per Hr |
| LCMS (without column, solvents & sample preparation) | 250 | 500 | 500 | 1000 | 2500 | 2500 | 2500 | 3750 | 3750 | 5000 | Per Hr |
| Protein Sequencing Identification LC MS-MS (Digested sample provide by user) | 300 | 600 | 600 | 1200 | 3000 | 3000 | 3000 | 4500 | 4500 | 6000 | Per Hr |
| Intact Mass of Protein, Peptide and Oligonucleotides (with solvent and sample preparation) | 75 | 150 | 150 | 300 | 750 | 750 | 750 | 1125 | 1125 | 1500 | Per Sample |
| Intact Mass of Protein, Peptide and Oligonucleotides (withot solvent and sample preparation) | 150 | 300 | 300 | 600 | 1500 | 1500 | 1500 | 2250 | 2250 | 3000 | Per Sample |
| Raw data soft copy | 50 | 100 | 100 | 200 | 500 | 500 | 500 | 750 | 750 | 1000 | Per Sample |
| Amount is in Rs. And 18% GST applicable | |||||||||||
Applications
- Qualitative Analysis & Quantitative Analysis.
- Proteins/Peptides:
In-depth qualitative analysis of complex samples.
Intact mass measurement.
- Small Molecules:
Identification and Characterization.
Metabolite analysis: targeted and non-targeted approaches.
Sample Details
MeoH, ACN, Water only
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