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Category
- Biocharacterization » Biochemistry
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
Used to study interaction between two molecules.
Isothermal titration calorimetry (ITC) works by measuring the heat change that occurs when two molecules interact. The principle behind ITC is that heat is released or absorbed when non-covalent bond from or break between two molecules. The heat change is measured by the instrument as heat required to maintain same temperature as that of reference cell.
- MicroCal Calorimeter Solution Package SYS80004 - PEAQ-ITC – Ultrasensitive PEAQ-ITC Microcalorimetery Cell with syringe tower and Control Unit for real-time operation and post-run data analysis.
- PEAQ-ITC Washing Module with detergent capability for cleaning cell and titration syringe.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
An appointment will be given as per queue and will be informed by email.
New users are requested to contact lab before registration.
Charges for Analytical Services in Different Categories
Please send an email to ashutoshk@iitb.ac.in for the charges.
Applications
Isothermal titration calorimetry has been used in a wide array of fields. In the old days, this technique was used to determine fundamental thermodynamic values for basic small molecular interactions. In recent years, ITC has been used in more industrially applicable areas, such as drug discovery and testing synthetic materials. Although it is still heavily used in fundamental chemistry, the trend has shifted over to the biological side, where label-free and buffer independent values are relatively harder to achieve.