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Category
- Spectroscopy and Spectrometry » Mass Spectrometry
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
Matrix-assisted laser desorption/ionization is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers and other macromolecules). It is used for the study of protein/peptide characterization, polymer analysis, glycan analysis, nucleic acids & small molecule analysis.
Matrix-assisted laser desorption ionization (MALDI) time-of-flight (TOF) mass spectrometry works by ionizing a sample with a laser beam striking on the sample mixed with matrix. The charged ions generated are then separated in the Time of flight tube based on their mass to charge ratio.
- Laser technology - 355 nm frequency tripled Nd:YAG SmartBeam laser
- Laser focus diameter - 10 µm - enables high spatial resolution imaging without pixel overlap.
- Resolving power - Wide mass range resolving power up to 26,000.
- Two Modes of operation - Linear mode for labile molecules or anything bigger than 5kDa. &Reflectron mode for small molecules and peptides.
- Ion source cleaning - Laser based self-cleaning.
- Target plate - 384 position target plate (~1 µL spot size).
- Specificity of Ionisation - Can analyse both positive & negative ions in the same spot.
- Compass software suite - Flex Control for quick and easy data acquisition & Flex Analysis for automatic and interactive data analysis.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
- An appointment will be given as per queue and will be informed by email.
- Online sample registration through the IRCC web page will be accepted for internal users. External users can contact through Email.
- The users will be informed about their date and time of the slot by email.
- If the user cannot come even after accepting the date & time of the slot, a mail should be immediately sent to maldi.bios@iitb.ac.in to cancel the slot.
- For re-allocating the slot after cancellation, fresh online registration to be done.
- Kindly refer the sample preparation instructions and do's & don'ts prior to filling up the online sample request form.
- Please come prepared with some literature reference papers for the sample to be analysed by MALDI TOF/TOF.
- After sample submission, the user need not stay back in the MALDI facility and the results will be sent through email.
- For samples other than Proteins, Peptides, Glycans, Nucleic acids & Polymers, kindly discuss with the concerned person at MALDI facility at 022 25764757, prior to online registration.
- Experiments should be discussed with the facility in-charge before appointment.
- Purity of samples is extremely important for generating good data.
- Protein concentrations should be measured accurately before starting the experiment.
- The molecular weight (SDS IMAGE copy mandatory)as well as the pI of the proteins should be known before analysis.
- Zip-tipping of samples must for all peptide analysis and also for proteins wherever required (will be confirmed by the MALDI staff in charge).
- Appointments will be provided as per que and the user will be informed about the same.
- Kindly perform literature review on similar work and accumulate as much information as possible for good quality data.
- For any further query, kindly contact on Email: maldi.bios@iitb.ac.in, Contact: 022 25764757
1. A requisition letter addressed to Head, BSBE along with a printout of electronic fund transfer proof should be sent by post or submitted in person to BSBE Department, Maldi lab, IIT Bombay, Powai, Mumbai-400076.
2. If you are sending samples by post please mention sample details:
a. Nature of sample
b. Expected Molecular weight
c. Used Matrix details
d. Maldi literature
3. Once we receive the requisition letter and advance payment, your appointment will be scheduled as per the queue.
4. The users will be informed about their date and time of slot by e-mail.
5. We prefer that you or your representative, who knows/understands the sample/material should be present on the day of appointment.
Charges for Analytical Services in Different Categories
Type of Sample | Type of Analysis | Charges (Rs.) per Sample | |||
| IITB | Academics | National R & D Labs | Industry | ||
Intact Protein | Molecular Weight Only | 200 | 1000 | 2500 | 4000 |
Peptide Mass Fingerprint (PMF) | Only MS | 200 | 1000 | 2500 | 4000 |
| MS/MS | 300 (5 PEAKS) | 2000 (5 PEAKS) | 5000 (5 PEAKS) | 5600 (5 PEAKS) | |
Small Molecules | Only MS | 200 | 1000 | 2500 | 4000 |
Oligonucleotides | Only MS | 200 | 1000 | 2500 | 4000 |
Applications
- Molecular weight determination of intact proteins
- Molecular weight determination of Glycans
- Molecular weight determination for Proteins & peptides
- Molecular weight determination for Nucleic Acids
- Polymer Characterization
Sample Details
SOP, Lab Policies and Other Details
Publications
- Joshi S, Khatri L K, Kumar A*, Rathore S A. Monitoring size and oligomeric-state distribution of therapeutic mAbs by NMR and DLS: Trastuzumab as a case study. J Pharm Biomed Anal. 2021. 195, 113841. doi: 10.1016/j.jpba.2020.113841
- Pravin N, Kumar R, Tripathi S, Kumar P, Mohite GM, Navalkar A, Panigrahi R, Singh N, GadheLG, Manchanda S, Shimozawa M, Nilsson P, Johansson J, Kumar A, Maji SK, Shanmugam M. Benzimidazole‐based Fluorophores for the Detection of Amyloid Fibrils with Higher Sensitivity than Thioflavin‐T. J. Neurochem. 2021. 156(6), 1003-1019. doi: 10.1111/jnc.15138
- Marathe S, Dhamija B, Kumar S, Jain N, Ghosh S, Dharikar JP, Srinivasan S, Das S, Sawant A, Desai S, Khan F, Syiemlieh A, Munde M, Nayak C, Gandhi M, Kumar A, Srivastava S, Venkatesh KV, Barthel SR, Purwar R. Multiomics Analysis and Systems Biology Integration Identifies the Roles of IL-9 in Keratinocyte Metabolic Reprogramming. J Invest Dermatol. 2021. doi: 10.1016/j.jid.2021.02.013.
- Debdeep Chatterjee, Reeba S Jacob, Soumik Ray, AmbujaNavalkar, Namrata Singh, ShinjineeSengupta, LaxmikantGadhe, Pradeep Kadu, DebalinaDatta, Ajoy Paul, SakunthalaArunima, SurabhiMehra, ChinmaiPindi, Santosh Kumar, PrafulSingru, SanjibSenapati, Samir K Maji. Co-aggregation and secondary nucleation in the life cycle of human prolactin/galanin functional amyloids. eLife. 2022. 11: e73835
- LaxmikantGadhe, ArunimaSakunthala, Semanti Mukherjee, NitishaGahlot, Riya Bera, Ajay Singh Sawner, Pradeep Kadu, Samir K. Maji. Intermediates of α-synuclein aggregation: Implications in Parkinson’s disease pathogenesis. Biophysical Chemistry. 2021. 281: 106736
- Soumik Ray, Nitu Singh, Komal Patel, G. Krishnamoorthy and Samir K. Maji. FRAP & FRET investigation of α-synucleinfibrillization via liquid-liquid phase separation in vitro and in HeLa cells. Methods in Mol Biol. 2021.
- SurabhiMehra ,LaxmikantGadhe, Riya Bera, Ajay Singh Sawner, Samir K Maji. Structural and functional insights into α-synuclein fibril polymorphism. Biomolecules. 2021. 11(10):1419
- AS Sawner, S Ray, P Yadav, S Mukherjee, R Panigrahi, M Poudyal, K Patel, D Ghosh, E Kummerant, A Kumar, R Riek and SK Maji. Modulating α-Synuclein Liquid–Liquid Phase Separation. Biochemistry. 2021. 60(48): 3676-3696