.
Category
- Biocharacterization » Cytometery
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Designed to sort cells at high flow speed The sorted cells can be collected in a varietyof vessels including 15ml Falcon tubes for 2-waysort, 5ml FACS tubes for 4-way or 2-way sort & 12 well, 24 well, 48 well & 96 well plates for plate sort. The collection vessels should contain media or buffer Lasers: FACS1 (4 lasers) -UV laser (355nm) Blue (488nm), Yellow Green (561nm) & Red laser (633nm) FACS2 (5 lasers) - UV laser (355nm) Blue (488nm), Yellow Green (561nm), Red laser (637nm) & Violet (405). Detectors: FACS1- 13 parameters can be detected including scatter properties. FACS2 – 20 parameters can be detected including scatter properties
FACS is a sophisticated high throughput instrument. It enables sorting of heterogeneous mixture of biological cells depending upon the specific light scattering characteristics and/or fluorescent characteristics of each cell in population.
A beam of light hits hydrodynamically focused sample stream. Combination of different filters and detectors collect the scatterd light from sample.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
The requisition form is available online.
Users should submit duly filled registration forms online only. Hard copies will not be accepted.
System-generated mail will be sent to user's Id about the date & time of the slot that has been allotted.
Type/Toxicity/Pathogenecity of the samples and precautions during sample analysis and sample disposal need should be discussed with the operator/TAs before booking the slot.
Samples should not be viscous.
Samples should not be prepared in Organic solvents (e.g. THF, Acetone, Hexane, Toluene etc)
Sample should be a single cell suspension.Clumping should be avoided. Clumps can block nozzle and can affect analysis.</li>
Minimum volume of sample required for acquisition is 500ul and Sample density should be approx. 1x106 cells or particles per ml for better analysis.
User should bring appropriate compensation controls required for the experiment.
The users are expected to bring their own samples for analysis.
The user should bring an unstained sample for each experiment.
User should reach on time and external users 30 minutes ahead of commencing the slot.
User should come with 10 percent bleach to put into the waste while discarding.
Sample must not be suspended in organic solvents /media.
Sample density should be 1x10 6 cells/particles per ml.
Sample volume should be 500ul at least.
Charges for Analytical Services in Different Categories
Applications
Apoptosis assays
Immuno-phenotyping
Intra-cellular staining
Cell viability assay
Sample Details
Only liquid single cell suspension samples are allowed
Not allowed
Samples are discarded using 10 percent bleach in ethanol.
Industrial samples must be discarded along with guidelines maintained in the respective project.
SOP, Lab Policies and Other Details
Publications
1. Tubulin-Binding 3,5-Bis(styryl)pyrazoles as Lead Compounds for the Treatment of Castration-
Resistant Prostate Cancer,Vivian W.Y. Liao, Anuradha Kumari, Rajeshwar Narlawar, Soma
Vignarajan, David E. Hibbs, Dulal Panda and Paul W. Groundwater, Molecular
Pharmacology June 1, 2020, 97 (6) 409-422; https://doi.org/10.1124/mol.119.118539
2. Poojari, Radhika; Sawant, Avishkar V; Kini, Sudarshan; Srivastava, Rohit; Panda, Dulal
(2020). Antihepatoma activity of multifunctional polymeric nanoparticles via inhibition of
microtubules and tyrosine kinases. Nanomedicine, 15(4), 381–396. 10.2217/nnm-2019-0349
3. Three-dimensional cryogel matrix for spheroid formation and anti-cancer drug screening
Archana Singh 1
, Prakriti Tayalia 1
J Biomed Mater Res A . 2020 Feb;108(2):365-376.
doi: 10.1002/jbm.a.36822. Epub 2019 Nov 7. IF 4.39
4. The Th9 axis reduces oxidative stress and promotes the survival of malignant T-cells in
cutaneous T-cell lymphoma patients. Mol Cancer Res . 2020 Apr;18(4):657-668. doi:
10.1158/1541-7786.MCR-19-0894.https://www.ncbi.nlm.nih.gov/pubmed/31996468 IF 5.2
5. A Navalkar, S Pandey, N Singh, K Patel, B Mohanty, S Jadhav, P Chaudhari and SK Maji (2020),
Direct evidence of cellular transformation by prion-like p53 amyloid infection. J. Cell Science,
134(11): 258316
6. S Ray, N Singh, R Kumar, K Patel, S Pandey, D Datta, J Mahato, R Panigrahi, A Navalkar, S
Mehra, L Gadhe, D Chatterjee, AS Sawner, S Maiti, S Bhatia, J Gerez, A Chowdhury, A Kumar,
R Padinhateeri, R Riek, G Krishnamoorthy and SK Maji, (2020), α‐Synuclein aggregation
nucleates through liquid-liquid phase separation
7. Patel, Y., Rai, D., Das, K., Dhandayuthapani, S., & Mehra, S. (2020). Ethanol in Combination
with Oxidative Stress Significantly Impacts Mycobacterial Physiology.
https://doi.org/10.1128/JB
8. Chandna, P., & Gundabala, V. (2021). Pillar based microfluidic approach to sorting of
microparticle mixtures at various particle ratios. Microfluidics and Nanofluidics, 25(1).
https://doi.org/10.1007/s10404-020-02406-7
9. Kar, N., Gupta, D., & Bellare, J. (2021). Ethanol affects fibroblast behavior differentially at
low and high doses: A comprehensive, dose-response evaluation. Toxicology Reports, 8,
1054–1066. https://doi.org/10.1016/j.toxrep.2021.05.007
10. Nirmala, J. Grace, Rachineni, K., Choudhary, S., Hosur, R. V., & Lopus, M. (2021).
Triphala polyphenols-functionalized gold nanoparticles impair cancer cell survival through
induction of tubulin dysfunction. Journal of Drug Delivery Science and Technology, 61.
https://doi.org/10.1016/j.jddst.2020.102167
11. Gupta, D. & Bellare, J. Highly controlled robotic customized gel functionalization on 3D
printed PCL framework for bone tissue engineering. Bioprinting24, (2021).
12. Surabhi Mehra, Sahil Ahlawat, Harish Kumar, Debalina Datta, Ambuja Navalkar, Nitu Singh,
Komal Patel, Laxmikant Gadhe, Pradeep Kadu, Rakesh Kumar, Narendra N Jha, Arunima
Sakunthala, Ajay S Sawner, Ranjith Padinhateeri, Jayant B Udgaonkar, Vipin Agarwal, Samir K
Maji, (2022), α-Synuclein aggregation intermediates form fibril polymorphs with distinct
prion-like properties, Journal of Molecular Biology 434(19), 167761
13. Arunima Sakunthala ,Debalina Datta,Ambuja Navalkar,Laxmikant Gadhe,Pradeep
Kadu,Komal Patel,Surabhi Mehra,Rakesh Kumar,Debdeep Chatterjee,Jyoti Devi,Kundan
Sengupta,Ranjith Padinhateeri and Samir K. Maji*,Direct Demonstration of Seed Size-
Dependent α-Synuclein Amyloid Amplification, J. Phys. Chem. Lett. 2022, 13, 28, 6427–6438,
July 11, 2022, Copyright © 2022 American Chemical Society
14. Ambuja Navalkar, Ajoy Paul, Arunima Sakunthala, Satyaprakash Pandey, Amit Kumar Dey,
Sandhini Saha, Sarthak Sahoo, Mohit K Jolly, Tushar K Maiti, Samir K Maji, (2022), Oncogenic
gain of function due to p53 amyloids occurs through aberrant alteration of cell cycle and
proliferation, Journal of Cell Science 135(15), jcs259500
15. Biswas, A. et al. Stabilization and fluorescence light-up of G-quadruplex nucleic acids using
indolyl-quinolinium based probes. Phys. Chem. Chem. Phys. (2022) ,
doi:10.1039/d1cp04718c
16. Biswas S*, Tikader B*, Kar S and Viswanathan, G Modulation of signaling cross-talk between
pJNK and pAKT generates optimal apoptotic response. PLOS Computational Biology 2022;
Oct 14;18(10):e1010626. Impact Factor : 4.53
17. Continuous Flow Synthesis of Substituted 3,4-Propylenedioxythiophene Derivatives,
Dattatray L. Tarange,Nagaraj Nayak,Anil Kumar*, Org. Process Res. Dev. 2023,
https://doi.org/10.1021/acs.oprd.2c00356
18. Process intensification of dendritic fibrous nanospheres of silica (DFNS) via continuous flow:
a scalable and sustainable alternative to the conventional batch synthesis, Karuna
Veeramani, Nagaraj Nayak,Neil R. Cameron , and Anil Kumar , React. Chem. Eng., 2023,
https://doi.org/10.1039/D2RE00405D
19. Reactive Oxygen Species and DNA Damage. ACS Omega. 2023 Jan 10;8(3):3221-3235. doi:
10.1021/acsomega.2c06691.
20. Saha R, Patkar S, Pillai MM, Tayalia P. 2023. Bilayered Skin Substitute Incorporating Rutin
Nanoparticles for Antioxidant, Anti-inflammatory and Anti-fibrotic Effect. Biomater.
Adv.150:213432.
21. Hemavathi Dhandapani, Armaan Siddiqui, Shivam Karadkar, Prakriti Tayalia. In vitro 3D
spheroid model preserves tumor microenvironment of hot and cold breast cancer
subtypes. Advanced Healthcare Materials. (accepted for publication on 29th April 2023)
22. Sagitha P, Dhandapani H, Tayalia P. Choline ester based ionic liquid: A multi-functional
system to enhance nucleic acid stability, drug solubilization and cell penetration. Int J Biol
Macromol. 2023 Mar 17;238:124059. doi: 10.1016/j.ijbiomac.2023.124059. Epub ahead of
print. PMID: 36934812.
23. V. K. Rao, A. Ashtam, D. Panda, S. K. Guchhait, ChemMedChem 2024, 19, e202300562.
https://doi.org/10.1002/cmdc.202300562
24. Giri, P., Batra, P. J., Kumari, A., Hura, N., Adhikary, R., Acharya, A., Guchhait, S. K., & Panda,
D. (2023). Development of QTMP: A promising anticancer agent through NP-Privileged
Motif-Driven structural modulation. Bioorganic & medicinal chemistry, 95, 117489.
https://doi.org/10.1016/j.bmc.2023.117489
25. Kurian, J., Ashtam, A., Kesavan, A., Chaluvalappil, S. V., Panda, D., & Manheri, M. K. (2023).
Hybridization of the Pharmacophoric Features of Discoipyrrole C and Combretastatin A-4
toward New Anticancer Leads. ChemMedChem, 18(16), e202300081.
https://doi.org/10.1002/cmdc.202300081
26. Pooja J Batra, Anuradha Kumari, Vivian W Y Liao, David E Hibbs, Paul W Groundwater, Dulal
Panda, 3,5-bis(styryl)pyrazole inhibits mitosis and induces cell death independent of BubR1
and p53 levels by depolymerizing microtubules, The Journal of Biochemistry, Volume 174,
Issue 2, August 2023, Pages 143–164, https://doi.org/10.1093/jb/mvad031
27. Mukherjee, S., Sawant, A. V., Prassanawar, S. S., & Panda, D. (2023). Copper-Plumbagin
Complex Produces Potent Anticancer Effects by Depolymerizing Microtubules and Inducing
Reactive Oxygen Species and DNA Damage. ACS omega, 8(3), 3221–3235.
https://doi.org/10.1021/acsomega.2c06691
28. Shubhank Sherekar, Chaitra S. Todankar & Ganesh A. Viswanathan Modulating the dynamics
of NFκB and PI3K enhances the ensemble-level TNFR1 signaling mediated apoptotic
response, npj Systems Biology and Applications volume 9, Article number: 57 (2023), 16
November 2023
29. Debalina Datta, Ambuja Navalkar, Arunima Sakunthala, Ajoy Paul, Komal Patel, Shalaka Mas
urkar, Laxmikant Gadhe, Shinjinee Sengupta, Manisha Poudyal, Jyoti Devi, Ajay
Singh Sawner, Pradeep Kadu, Ranjit Shaw, Satyaprakash Pandey, Semanti Mukherjee, Nitisha
Gahlot, Kundan Sengupta, Samir K Maji, Nucleo-cytoplasmic environment modulates spatio-
temporal p53 phase separation, bioRxiv. October 18, 2023
30. Anjali Mahilkar, Prachitha Nagendra, Pavithra Venkataraman, Saniya Deshmukh, Supreet Sai
ni , Rapid evolution of pre-zygotic reproductive barriers in allopatric populations, ASM
Journals,Microbiology Spectrum,Vol. 11, No. 6, 3 October 2023
https://doi.org/10.1128/spectrum.01950-23
31. Niyati Piplani , Tanusri Roy , Neha Saxena , Shamik Sen * Dept. of Biosciences &
Bioengineering, IIT Bombay, Mumbai, India, Bulky glycocalyx shields cancer cells from
invasion-associated stresses, journal homepage: www.elsevier.com/locate/tranon,
Translational Oncology, January 2024
32. Resolving the backbone tilt of crystalline poly(3-hexylthiophene) with resonant tender X-ray
diffraction, Guillaume Freychet,
a
Paul Chantler,b
Yuxuan Huang,c
Wen Liang
Tan,b
Mikhail Zhernenkov,
a
Nagaraj Nayak,d
Anil Kumar,
d
Peter A. Gilhooly-
Finn,ef
Christian B. Nielsen,
e
Lars Thomsen,g
Subhayan Roychoudhury,h
Henning
Sirringhaus, David Prendergast and Christopher R. McNeill, Mater. Horiz., 2022,
https://doi.org/10.1039/D2MH00244B