Two-dimensional Gas Chromatography with time of flight mass spectrometer (GC-GC-TOF-MS) system with gerstel auto sampler can facilitate high end separation of volatile organic compounds in highly complex mixtures and environmental samples. The two-dimensional output helps in resolving the peak of target analytes even when matrix interferences are present. The enhanced spectral collection rate of the TOF-MS detector together with the software capabilities can help in de-convolution of co-eluting compounds.
Request form for external booking (Sample and analysis details)
Sample_Proforma_GC_GC_TOF_MS_ext_2018-02-09.pdf (90.28 KB)
Make
LECO
Model
Pegasus 4D with Agilent 7890B GC
Facility Status
Working
Date of Installation
Facility Management Division
Institute Central Research Facilities (ICRF)

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Category

  • Chromatography » Gas Chromatography
  • Spectroscopy and Spectrometry » Mass Spectrometry

Booking Details

Slot Booking Link
Booking link for external users
Booking link for IIT Bombay internal users
Booking available for
Internal and External Both
Available Equipment/ Mode of use
Liquid/gas injection
Cooled injection system and thermal desorption unit (CIS-TDU)
Headspace injection
Stir bar sorptive extraction (twister)
solid phase micro-extraction (SPME)
Pyrolysis

Facility Management Team and Location

Faculty In Charge
Prof. Suryendu Dutta, s.dutta@iitb.ac.in, +91-22-2576 7278
Facility Manager
Ms. Mursaleen Shaikh, mursaleen@iitb.ac.in, 022 2159 6376
Facility Operator
Ms. Mursaleen Shaikh, mursaleen@iitb.ac.in, 022 2159 6376
Facility Management Members
Prof. Suparna Mukherji
Prof. P P Wangikar
Prof. Seethamraju Srinivas
Prof. Subramaniam Chandramouli
Prof. Suryendu Dutta
Head, Centre for Research in Nanotechnology and Science(CRNTS)
Department
CRNTS
Lab Email ID
gcgctofms@iitb.ac.in
Facility Location
Room No. 114, Ground floor, Centre for Research in Nanotechnology and Science
Lab Phone No
022 2159 6376

Facility Features, Working Principle and Specifications

Features Working Principle

Features:

  • High resolution gas chromatographic separation can be achieved for complex samples, when operated in 2-D mode.
  • Independent temperature programming for each of the columns possible
  • MS can collect > 20 full range spectra across a 50ms GC peak
  • High dynamic range can be achieved

Working Principle:

The GC-GC-TOF-MS facility was installed in Centre for Research in Nanotechnology and Science (CRNTS) in January 2016 as a Central Facility as per RIFC norms. The facility is available for meeting the research needs of all users.

It can perform all analysis that a typical GC-TOF-MS can perform. Its particular benefit is for analysis of organic compounds from complex mixtures and environmental samples where high end separation is involved. It involves two columns (that can achieve separation based on different attributes). A thermal modulator ensures cryo focusing of effluent from the first column before its release to the second column. The two-dimensional output helps in resolving the peak of target analytes even when matrix interferences are present. The enhanced spectral collection rate of the TOF-MS detector together with the software capabilities can help in de-convolution of co-eluting compounds.

Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures

Instructions for Registration
  • Analysis should be discussed with facility in-charge before proceeding. Prior literature review for similar sample analysis is recommended for better quality results
  • Registration is online (for internal users) through the Drona interface.
  • An appointment will be given as per queue and will be informed by email.
  • If an appointment is given but the user cannot come an e-mail should be immediately sent to gcgctofms@iitb.ac.in to cancel or postpone his/her slot.
Instruction for Sample Preparation
  • The sample should be dissolved in volatile organic solvents, preferably hexane or isooctane.
  • Other solvents like, methanol, ethanol, ethyl acetate etc. can also be used.
  • Solvents such as DMSO, THF and water are not acceptable.
  • The sample should be free of particulates of size > 0.45 μm
User Instructions and Precautionary Measures
  • One sample refers to one injection in any of these modes. Multiple injections of the same sample will be treated as multiple samples. Delays may be involved if injector change is required for sample analysis.
  • The analytes expected in the samples should be clearly indicated and MSDS (Material Safety Data Sheet) should be given both for the samples and the analytes to indicate the toxicity.
  • Charge for 2-dimensional (D) analysis will be same as 1-D; However no samples will be directly analyzed in 2-D mode before a 1-D run has been conducted. For similar type of samples, after 1 1-D run is done the remaining can be allowed directly in 2D.
  • The users will be informed about their date and time-slot by email for submission of sample/s. If the day and time-slot is not suitable for you, an email request should be sent immediately for an alternate slot.
  • Samples are to be brought along on the date of your appointment for submission. The samples will be analyzed on the next day of submission.
  • After the sample analysis is complete the results will be sent through email. For large data files user may provide a blank CD for the data files.
  • Users desiring to get back the samples need to mark on the form. Collect them from the lab between 9:30 AM to 11:00 AM after two-three days of completion of the work (intimated through mail) and uncollected samples will be disposed-off after ten days of intimation of the completion of the work and no reminders will be sent.

Charges for Analytical Services in Different Categories

Applications

  • Environmental.
  • Geochemistry
  • Biological
  • Chemical/Chemistry

Sample Details

SOP, Lab Policies and Other Details

Publications

Publications:

  1. Kumar, S., Dutta, S., Bhui, U.K., 2021. Provenance of organic matter in an intracratonic rift basin: insights from biomarker distribution in Palaeogene crude oils of Cambay Basin, western India. Organic Geochemistry 162, 104329
  2. Bhattacharya, S., Dutta, S., Kumar, S., 2021. Identification of lanostanes, A-ring methylated steranes and secosteranes in late Neoproterozoic crude oils by GC×GC-TOFMS: New insights into molecular taphonomy of steroids. Geobios68, 47-59
  3. Dhiman, H., Dutta, S., Kumar, S., Verma, V., Prasad, G.V.R., 2021. Discovery of proteinaceous moieties in Late Cretaceous dinosaur eggshell. Palaeontology 64, 585-595.
  4. Mishra, S., Dutta, S., Singh, V.P., Kumar, S., Mathews, R.P., Jha, N., 2021. A new acritarch spike of Leiosphaeridiadessicata comb. nov. emend. from the Upper Permian and Lower Triassic sequence of India (Pranhita-Godavari Basin): Its origin and palaeoecological significance. Palaeogeography, Palaeoclimatology, Palaeoecology 567, 110274.
  5. Menon, N.G., Tatiparti, S.S.V. and Mukherji, S. (2021) “Efficacy and reusability of mixed-phase TiO2- ZnO nanocomposites for the removal of estrogenic effects of 17β-estradiol and 17α-ethinylestradiol from water” Journal of Environmental Management, 288:112340.
  6. Chand, P. Dutta, S. and Mukherji, S. (2022) “Characterization and Biodegradability Assessment of the Water Accommodated Fraction of Oily Sludge Using Stir Bar Sorptive Extraction and GCxGC-TOF-MS” Environmental Pollution, 304, 119177.
  7. Umamaheswaran, R., Dutta, S., Khan, M.A., Bera, M., Bera, S., Kumar, S., 2022. Identification of Chitin in Pliocene Fungi Using Py-GC×GC-TOFMS: Potential Implications for the Study of the Evolution of the Fungal Clade in Deep Time. Analytical Chemistry 94, 1958-1964.
  8. Umamaheswaran, R., Dutta, S., Singh, H., Kumar, S., 2022. Pyrolysis-GC×GC-TOFMS as a tool for distinguishing the macromolecular structure of nitrogen-bearing animal biopolymers in fossil tissues. Journal of Analytical and Applied Pyrolysis 161, 105362.
  9. Chand, P., Dutta, S., & Mukherji, S. (2022). Slurry phase biodegradation of heavy oily sludge and evidence of asphaltene biotransformation. Journal of Environmental Management, 324, 116315.
  10. Ghosh, S., Dutta, S., Bhattacharyya, S., Konar, R., & Priya, T. (2022). Paradigms of biomarker and PAH distributions in lower Gondwana bituminous coal lithotypes. International Journal of Coal Geology, 260, 104067. https://doi.org/10.1016/j.coal.2022.104067
  11. Umamaheswaran, R., Dutta, S., Prasad, G. V. R., Khan, M. A., Kumar, S., Bera, S., & Patnaik, R. (2022). The diagenetic fate of collagen as revealed by analytical pyrolysis of fossil fish scales from deep time. Geobiology. https://doi.org/10.1111/gbi.12537
  12. Kanduri, P. K., & Seethamraju, S. (2022). In situ and ex situ catalytic co-pyrolysis of lignocellulosic biomass and plastics (Low-Density and High-Density polyethylene) using spent FCC catalyst. Waste and Biomass Valorization, 14(5), 1737–1751. https://doi.org/10.1007/s12649-022-01961-0
  13. Kanduri, P. K., & Seethamraju, S. (2023). Effect of process mode on product yields and selectivity in the catalytic co-pyrolysis of peanut shells and polypropylene using spent fluid catalytic cracking (FCC) catalyst. Bioresource Technology Reports, 22, 101478. https://doi.org/10.1016/j.biteb.2023.101478
  14. Umamaheswaran, R., & Dutta, S. (2024). Preservation of proteins in the geosphere. Nature Ecology and Evolution. https://doi.org/10.1038/s41559-024-02366-z

Conferences

  1. Mukherji, S., Chand, P. and Datta S. “Slurry phase Biodegradation of Aged Oily Sludge from a Refinery by Indigenous Bacteria and Insights Gained from GCxGC TOF MS Analysis” International Conference on Biotechnology for Sustainable Agriculture, Environment and Health (BSAEH-2021), Jaipur, India, 4th-8th April 2021. Invited Oral Presentation.
  2. Mukherji, S., Jasmine, J. Chand, P. and Dutta, S. “Perspectives on Oily Tank Bottom Sludge and its Slurry Phase Treatment using Indigenous Microbes”, 4th Virtual Conference on “Waste Management Technology Trend and Developments” Organized by MattCorr, 19th and 20th August 2021.
  3. Niyolia, D., Dutta, S., Samant, B., 2021. Biomarker Signatures in Cretaceous Sediments of an Island Continent. 30th International Meeting on Organic Geochemistry, Virtual Conference. DOI: https://doi.org/10.3997/2214-4609.202134039.
  4. Ghosh, P. and Mukherji, S. “Development of Methods for Identification and Quantification of Selected PAHs, Heterocyclics, and their Degradation Products” International Conference on Environmental Science & Engineering, Organized by Environmental Science & Engineering Department, IIT Bombay, 20th-22nd January 2022. Poster Presentation.
  5. Mukherji S. “Removal of Pharmaceuticals and Pesticides from Water Using Photocatalytic Nanocomposites” Invited Presentation in Half day Symposium on “Detection and Remediation of Emerging Water Contaminants” organized by Department of Instrumentation and Control Engineering, Manipal Institute of Technology (MIT), Manipal, India, 1st March, 2022
  6. Mukherji S. “Opportunities and Challenges in Photocatalytic Removal of Micropollutants and Emerging Contaminants” 11th Young Environmental Scientists (YES) Meeting, Society for Environmental Toxicology and Chemistry (SETAC), 7th-9thMarch 2022. Virtual Conference. Keynote Lecture.
  7. Umamaheswaran, R., Dutta, S., Kumar, S., & Bera, S. (2022). A novel analytical pyrolysis technique for the assessment of chitinous traces in putative fungal fossils, at The 6th International Palaeontological Congress held at Thailand.
  8. Ghosh, S., Dutta, S., Bhattacharyya, S., & Priya, T. (2022). Biomarker and PAH Niceties in Early Permian Coal Lithotypes, at 73rd Annual Meeting and Symposium of ICCP at New Delhi.
  9. Priya, T., Dutta, S., & Prasad, V. (2022). Paleodiversity of flora from the Early Paleogene of North-East India: an organic geochemical approach, at The 6th International Palaeontological Congress held at Thailand
  10. Priya, T., Dutta, S., & Prasad, V. (2023) Early Palaeogene vegetation from the tropics: Insights from molecular fossils, at Frontiers of Geoscience Research conference, held at PRL Ahmedabad.
  11. Priya, T., Dutta, S. & Prasad, V. 2023. Organic Geochemical Insights into the early Palaeogene vegetation from the Tropics. 31st International Meeting on Organic Geochemistry (IMOG), Montpelliar, France.
  12. Chand, P., Dutta S. and Mukherji, S. "Detection and elucidation of fate of organic contaminants in the water-soluble fraction of oily sludge using GCxGC TOF MS" International Conference on Advanced Materials and Characterization, organized by SG Lab Forum, Singapore, April 25th-26th and 28th 2023.  Oral Presentation.
  13. Chand, P., Dutta, S. and Mukherji, S. (2023) "Analysis of oily sludge and its WSF and estimation of component contribution to toxicity using the Petrotox model" Symposium on Improving Water Quality by Understanding Environmental Chemical Processes, ACS Fall Meet, San Francisco, CA, USA, 13th to 17th August 2023. Oral Presentation.