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Category
- Chromatography » Liquid Chromatography
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
Preparative Chromatography is carried out under low backpressures with bigger inner diameter columns and bigger particle size by passing mobile phases at high flow rates. It is used to purify and collect component of interest from all other contaminants in sample.
- It can be used for analytical as well as Prep analysis depending upon column used and mode selected.
- LC-20AP Pump along with FCV-200AL unit (Quaternary valve) is capable of using 4 solvents with max. flow up to 50mL/ min.
- Autosampler LH-40 Liquid Handler is available for Analytical and prep mode which introduces a precise aliquot of a sample solution from a sample container to HPLC column. It also works as the fraction collector which collects fractions even when the peak elution time varies, by catching the target component according to variations in the chromatogram.
- PDA detector (SPD-M40): It can detect entire Wavelength range of 190 nm - 800 nm simultaneously, wavelength accuracy ± 1 nm.
Following analytical column are available with system Shim-pack GIS G C18 5um 4.6 X 250, Shim-pack UC Sil (250 X 4.6,5um).
- LCMS 2020 combines fast scan speeds with ultrafast polarity switching generates an LCMS/MS instrument that can rapidly analyse numerous compounds with m/z 10 to 2,000.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
User must contact technical operator before booking the slot and discuss about experiment parameters.
Only solvents compatible with the column can be used. All solvents and any chemicals required to be provided by the user.
- User is expected to know the details about method and column to be used. Method suitable for column also to be provided by the end user.
- Internal Users are supposed to bring all chemicals required for the experiment.
Charges for Analytical Services in Different Categories
Charges in INR
Analysis | Chemistry Department Users | IITB users | Academic Institutions | National R & D Labs | Industries/ Non Govt. Agencies |
Basic Charges per sample (each single recording) for analytical mode |
Rs. 50/- |
Rs. 100/-
|
Rs. 750/- |
Rs. 1500/- |
Rs. 3000/- |
Basic Charges for Prep mode run per hour of sample runtime |
Rs. 100/- |
Rs. 150/-
|
Rs. 200/- |
Rs. 2500/- |
Rs. 5000/- |
For external samples in addition to basic charges, prevailing GST will be added.
Note:
These are only instrument charges. Chemicals/ reagents/ solvents required for analysis will be supplied by end user.