External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
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Category
- Synthesis » Biochemical Synthesis
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
It is used to prepare Solid-phase peptide synthesis (SPPS) which involves the successive addition of protected amino acid derivatives to a growing peptide chain immobilized on a solid phase, including deprotection and washing steps to remove unreacted groups and also side products.
Specification / Features:
1. Scale of Synthesis: 0.005 - 1.0mmol per reaction vessel (Maximum 2 Reaction Vessels at a time)
2. Heating method: Controlled induction heating, configurable to the number of RVs
3. Agitation method: Argon bubbling - adjustable, and/or option oscillating shaker mixing
4. Reaction vessel volume: Plastic, disposable: 10 mL Borosilicate glass: 10 mL, 25 mL, 40 mL
5. Number of solvent positions/sizes: upto 8 solvents
6. Number of amino acid positions: 27 amino acid bottles positions
7. Control interface: Proprietary PurePep Chorus control software, internal CPU, tablet (wireless connection)
It is used to prepare Solid-phase peptide synthesis (SPPS) which involves the successive addition of protected amino acid derivatives to a growing peptide chain immobilized on a solid phase, including deprotection and washing steps to remove unreacted groups and also side products.
Sample Preparation, User Instructions and Precautionary Measures
- Chemicals/Reagents/ solvents are to be supplied by end user.
- If end users do not supply chemicals, reagents/solvents, in addition to above basic charges, there will be additional charges as per the peptide sequence. The end-user will be provided a quotation for the same.
- User Should give detailed information about method, Resin, Chemicals to be used.
- Chemicals/Reagents/ solvents are to be supplied by end user.
- Internal User must get trained before using this Instrument.
- Additional Nitrogen gas charges: Rs 750 per day (Minimum charges are Rs 750)
- If end users do not supply chemicals, reagents/solvents, in addition to above basic charges, there will be additional charges as per the peptide sequence. The end-user will be provided a quotation for the same.
Charges for Analytical Services in Different Categories
.Charges:
| IITB (TAs) | IITB Students | IITB-Monash Students | Academic Institutes | National Labs | Sine (letter from SINE reqd.) | Research Park (MSME) (letter from RP reqd.) | Research Park (Big Industry partners) (letter from RP reqd.) | and MSME not associated with RP (appropriate certificate required) | Industries | |
| Charges | X/2 | X | X | 2X | 5X | 5X | 5X | 7.5X | 7.5X | 10X |
| DNA/ RNA Synthesizer (BIOSYNTH-02) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| Peptide Synthesizer (BIOSYNTH-03) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| Nitrogen charges for Peptide synthesizer per day additional | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 | 750 |
| HPLC(BIOSYNTH-04)per hour including pre and post washtime | 125 | 250 | 250 | 500 | 1250 | 1250 | 1250 | 1875 | 1875 | 2500 |
| Freeze Dryer (BIOSYNTH-05) per day | 500 | 1000 | 1000 | 2000 | 5000 | 5000 | 5000 | 7500 | 7500 | 10000 |
| GST | NO GST | NO GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST | 18 % GST |
All charges in INR
Additional Nitrogen gas charges: Rs 750 per day (Minimum charges are Rs 750)
These are only Instrument recording charges. Chemicals/Reagents/ solvents are to be supplied by end user. For external samples in addition to above basic charges, prevailing GST will be added
Applications
Sample Details
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