Nuclear magnetic resonance spectrometer- 500 MHz Facility
Nuclear magnetic resonance spectrometer- 500 MHz
Make
BRUKER. Switzerland
Model
AVANCE III HD
Facility Status
Working
Facility Management Division
Institute Central Research Facilities (ICRF)

Category

  • Spectroscopy and Spectrometry » NMR Spectroscopy

Booking Details

Booking available for
Internal and External Both
Available Mode for Use
1D experiments eg 1D NOE, APT, DEPT can be recorded 2D experiments eg COSY, NOESY, TOCSY, HSQC, HMBC can be done. Solid state samples for 13C, 31P etc nuclei can be recorded. Variable temperature experiment can be recorded

Facility Management Team and Location

Faculty In Charge
Prof. Santosh J. Gharpure
Co-convenors
Prof. Santosh J. Gharpure
Prof. P Ghosh
Prof. Ashutosh Kumar
Prof. Rajdip Bandyopadhyaya
Prof. A V Mahajan
Prof. Suvarn S. Kulkarni
Department
Chemistry
LAB Email ID
nmr500@iitb.ac.in
Facility Location
Room No. CL-16, Gr. Floor, Chemistry Department I.I.T. Bombay, Powai, Mumbai - 400076
Lab Phone No
0222576418

Facility Features, Working Principle and Specifications

Features Working Principle
  • Along with the routine 1D experiment of nuclei like 1H, 13C, 19F,31P, other nuclei 11B,27Al, 77Se,125Te, 199Hg etc can be recorded.
  • 1D experiments eg 1D NOE, APT, DEPT can be recorded
  • 2D experiments eg cosy, noesy, tocsy, HSQC,HMBC can be done.
  • Low temp and high temp experiments can be performed.
  • Solid state samples for 13C, 31P etc nuclei can be recorded.
  • The principle behind NMR is that many nuclei have spin and all nuclei are electrically charged. If an external magnetic field is applied, an energy transfer is possible between the base energy to a higher energy level (generally a single energy gap). The energy transfer takes place at a wavelength that corresponds to radio frequencies and when the spin returns to its base level, energy is emitted at the same frequency. The signal that matches this transfer is measured in many ways and processed in order to yield an NMR spectrum for the nucleus concerned.
Body Specification
  • Variable temperature facility is available. Samples can be recorded in the temperature range from -40°C to + 60°C with suitable solvent.
  • Along with the routine 1D experiment of nuclei like 1H, 13C, 19F, 31P, other nuclei 11B, 27Al, 77Se, 125Te, 199Hg etc can be recorded.
  • With 5mm multinuclear probe (BBFO probe), analysis of liquid samples of nuclei like 1H, 31P, 13C, 77Se, 125Te, 199Hg etc can be recorded.
  • PROBES :
    • 5mm multinuclear probe for solution studies (Broadband probe)
      For nuclei like 1H, 11B, 13C, 31P, 19F , 77Se , 125Te , 199Hg etc.
    • In case of solids, only broad band frequency range is available and the sample can be spun up to a maximum of 15 KHz.
  • VT ACCESSORY : Variable temperature facility is available. Currently Samples can be recorded in the temperature range from -40°C to + 60°C with suitable solvent.

Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges

Instructions for Registration
  • External Users can come in-person or send your samples along with a letter from the Head / Guide on your College/Institute /Industry Original Letter Head for registration stating that the analysis is for research purpose to qualify for academic concession. The letter should be addressed to The Head, Dept. Of Chemistry, IIT, Bombay.
  • The analytical data / spectra are provided only for research / development purposes. These can not be used as certificates in legal disputes.
Instruction for Sample Preparation
  • Uers are advised to provide 10-20 mg of sample depending upon its molecular weight for obtaining high resolution proton NMR spectra.
  • The sample should be completely soluble in 0.6 cc of the given solvent.
  • For 13C at least 20-30 mg of sample is necessary for obtaining spectra with reasonable signal / noise ratio. However, higher amount of sample helps in reducing data acquisition time which in turn leads to reduction of analysis charges.
  • For MAS-NMR experiments, sample should be in the form of very fine powder (20 microns) and at least 100-200mg of powder is required.
  • All external users are required to submit samples accompanied by a letter containing analysis details.
  • While requesting for analysis, information on i) type of analysis, ii) solvent and iii) spectral range should be given clearly in the letter.
  • For any query, please contact NMR Lab, ground floor Chemistry Dept. 
    Contact no. 022-25764181 or 022-25767151 ( Chemistry office)
Charge Details
Screenshot from 2024-08-08 16-59-38.pdf (96.36 KB)

Applications

  • Major application of NMR spectroscopy lies in the area of synthetic organic chemistry, inorganic chemistry, bio-organic chemistry, bio-inorganic chemistry, polymer chemistry and organometallic chemistry.One of the main advantages of FT-NMR spectroscopy is that a number of FIDs can be accumulated and Fourier transformed, enhancing S/N ratio of the spectrum. This in turn facilitates the analysis of samples with low abundant nuclei and smaller gyro-magnetic ratios, e.g.,13C, 29Si, 15N etc.
  • NMR studies find applications in some of following areas :
  • Molecular conformation in solution
  • Quantitative analysis of mixtures containing known compounds
  • Determining the content and purity of a sample
  • Through space connectivity (overhauser effect)

Sample Details

SOP, Lab Policies and Other Details

Publications

Publications
NA