Field Emission Gun-Transmission Electron Microscope Facility (FEG-TEM 200 kV)
Request form for external booking (Sample and analysis details)
FEG-TEM200kV_MSDS_0.pdf (164.84 KB)
Make
JEOL
Model
JEM 2100F
Facility Status
Working
Date of Installation
Facility Management Division
Institute Central Research Facilities (ICRF)

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Category

  • Microscopy and Imaging » Electron Microscopy

Booking Details

Slot Booking Link
Booking Link for IIT Bombay Internal Users
Booking Link for External Users
Booking available for
Internal and External Both
Available Equipment/ Mode of use
TEM Bright field/dark field imaging
HR-TEM imaging
Diffraction pattern

Facility Management Team and Location

Faculty In Charge
Prof. Suparna Mukherji Head, Centre for Research in Nanotechnology and Science(CRNTS), mitras@iitb.ac.in, 7854
Co-convenors
Prof. Rajdip Bandyopadhyaya
Prof. Mohammed Aslam
Prof. Sankara Sarma V. Tatiparti
Facility Manager
Dr. Bharati Patro, bharati@iitb.ac.in , 6864/6865
Facility Operator
Mr. Anand Mehta, 30004841@iitb.ac.in , 022-2159 6865
Mrs. Aradhana P. Naudiyal, aaradhana@iitb.ac.in ,022-2159 6865
Facility Management Members
Prof. Suparna Mukherji
Prof. Rajdip Bandyopadhyaya
Prof. Mohammed Aslam
Prof. Sankara Sarma V. Tatiparti
Department
CRNTS
Lab Email ID
fegtemlab@iitb.ac.in
Facility Location
Room No: 315 - A Ground floor, CRNTS/SAIF
Lab Phone No
022-2159 6865

Facility Features, Working Principle and Specifications

Features Working Principle

Working Principle: The 200 kV Field Emission Gun-Transmission Electron Microscope (FEG-TEM) has the ability to form images that provide detailed information about the materials and can reveal the atomic arrangements up to a point resolution of 0.19 nm at localized regions. It also gives information about specimen crystallinity and its orientations through diffraction patterns. This electron microscope is also equipped with an electrode short switch, allowing for operation at 120 kV for observation and imaging of beam-sensitive specimens like biological, polymers etc.

Features:

  • EM-20014 ultrahigh resolution 
  • Point resolution : 0.19 nm 
  • Lattice resolution : 0.1 nm
  • Magnification : 50 x to 1,500,000 x 
  • Acc. voltage : 120 kV, 200 kV
  • Electron gun emitter : ZrO/W(100)
  • Brightness : ≥4x108  A/(cm2.sr) 
  • Pressure: On the order of 10-8 Pa 
  • Probe current : >=0.5 nA for 1 nm probe 

Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures

Instructions for Registration
  • New users are requested to contact the FEG TEM 200kV lab before registration. 
  • Registration is online (for internal users) through the Drona interface.
  • An appointment will be given as per queue and will be informed by email.
  • Cancellation/postponing a slot at least 24 hrs earlier.
Instruction for Sample Preparation
  • The user has to collect TEM grids from the FEG TEM lab.
  • The user has to come for TEM analysis with a prepared sample on the TEM grid.
  • The samples should be prepared on TEM grids of 3mm size and sample thickness should be less than 100 nm for high-resolution images.
  • Any query related to your FEG-TEM analysis can be emailed to fegtemlab@iitb.ac.in.
  • The samples should be dry and should withstand ultra-high vacuum.
User Instructions and Precautionary Measures

Charges for Analytical Services in Different Categories

Applications

  • Nano science/Nano Technology 
  • Micro/Nano electronics 
  • Thin Films 
  • Catalysis 
  • Corrosion 
  • Polymer Science 
  • Energy Science/Engg. 
  • Biological and life sciences 

Sample Details

Chemical allowed

N.A.

Allowed Substrate

N.A.

Gases allowed

N.A.

Substrate Dimension

N.A.

Target dimension

Sample thickness should be less than 100 nm for HR imaging

Contamination remarks

If samples are not dried, column vacuum gets contaminated

SOP, Lab Policies and Other Details

SOP
FEG-TEM 200kV SOP.pdf (1.18 MB)
Training and Other Policy Documents
FEG TEM 200kV TA policy.pdf (192.54 KB)

Publications

Publication Details:

Patents:

1. Rohit Srivastava and co-workers Dual therapeutic disintegrable GNR-Liposome nanohybrid for Plasmon is photo thermal Cancer theranostic: IDF: 10-04-17

2. Rohit Srivastava and co-workers Red emissive photo triggered Plasmonic nano-rods for deep tissue visualisation and localized tumour ablation: IDF: 03- 11-17

 3. Rohit Srivastava and co-workers Biodegradable fluorescent liposomal Nano composites and method of preparation thereof: IDF: 03-11-2017 

4. Rohit Srivastava and co-workers Multistimuli bioresponsive fluorescent annotated hollow mesoporous silica for cancer Theranostics : IDF: 03-11-2017 

5. Rohit Srivastava and co-workers Room temperature synthesis of biocompatible porous silica nanoparticles using lipid as a structure directing agent: IDF: 04- 12-2017 

6. Rohit Srivastava and co-workers Rapid and scalable microwave assisted hydrothermal method for synthesis of fluorescent carbon spheres: IDF: 07-02- 2018 

7. Rohit Srivastava and co-workers Polycaprolactone based Plasmon is nanoshells and applications thereof: IDF: 17-02-2018 

8. Rohit Srivastava and co-workers Red emissive liposomal nanopitchers for localised diagnosis and phototriggered tumour ablation: IDF: 13-03-2018 

9. Rohit Srivastava and co-workers One step biocompatible frolic acid stabilised end-end assembled gold nanorods and usage thereof: IDF: 21-03-18

 

10. Rohit Srivastava and co-workers Rapid and scalable bioresponsive plasmonic gated functional nanohybrid and their use of, US Patent, 20-16-18 

11. Rohit Srivastava and co-workers One-step scalable design of biocompatible gold nanorods for nanomedicine, US Patent, 20-16-18 

12. Kumawat, Mukesh Kumar; Thakur, Mukeshchand; Gurung, Raju B; Srivastava, Rohit; Process for the synthesis of multi-fluorescent carbon nanostructures. Indian Patent Application (IPA no. 201621011794)

 13. Kumawat, Mukesh Kumar; Thakur, Mukeshchand; Gurung, Raju B; Srivastava, Rohit; Red luminescent graphene quantum dots, synthesis and applications thereof. Indian Patent Application (IPA No. 201721016198) 

14. Chauhan, Deepak Singh; Kumawat, Mukesh Kumar; Srivastava, Rohit. Near infra-red hybrid nanomaterials and graphene oxide for theranostic applications. Indian Patent Application (IPA no. 4747/MUM/2015) 

15. Lakkakula, J. R.; Tharayil, Deepika Divakaran; Thakur, Mukeshchand; Kumawat, Mukesh Kumar; Srivastava, Rohit. Cyclodextrin functionalized inorganic nanostructures: synthesis and applications thereof. Indian Patent Application (IPA No. 201721016954)

 16. Bahadur, Rohan; Thakur, Mukeshchand; Kumawat, Mukesh Kumar; Srivastava, Rohit. Method for preparing graphene quantum dots (GQDs) and uses thereof. Indian Patent Application (IPA No. 201721038918)   

 

 

 

 

*Publications:*

 1. Fluorescence Stability Study of MPA-CdTe Quantum Dots in Various Biochemical Buffers. Vivek Borse, A Kashikar, R Srivastava Journal of Nanoscience and Nanotechnology 18, 2582–2591, 2018 

2. N-doped multi-fluorescent carbon dots for ‘turn off-on’ silver-biothiol dual sensing and mammalian cell imaging application. Vivek Borse, M Thakur, S Sengupta, R Srivastava. Sensors and Actuators B: Chemical 248, 481-192, 2017 

3. CdTe quantum dots: aqueous phase synthesis, stability studies and protein conjugation for development of biosensors. Vivek Borse, M Sadawana, R Srivastava. Nanophotonics VI, SPIE Photonics Europe 2016 9884 (988423), 2016 

4. ‘Turn-on’ fluorescence assay for inorganic phosphate sensing Vivek Borse, P Jain, M Sadawana, R Srivastava. Sensors and Actuators B: Chemical 225, 340–347, 2015 

5. Rohit Srivastava and co-workers, Disintegrable NIR light triggered gold nanorods supported liposomal nanohybrids for cancer theranostics, Bioconjugate Chem., 2018, 29, 1510-1518 

6. Rohit Srivastava and co-workers, NIR light-triggered shrinkable thermoresponsive PNVCL nanoshells for cancer theranostics, RSC.ADV., 2017, 7, 44026-44034 

7. Rohit Srivastava and co-workers, Facile synthesis of plasmonic zein nanoshells for imaging-guided photothermal cancer therapy, Mater. Sci. Engg. C, 2018, 90, 539-548 

8. Rohit Srivastava and co-workers, Plasmonic Carbon Nanohybrids for Repetitive and Highly Localized Photothermal Cancer Therapy, Colloids Surf B Biointerfaces, 2018

 

9. Rohit Srivastava and co-workers, Biodegradable fluorescent nanohybrid for photo-driven tumor diagnosis and tumor growth inhibition, Nanoscale. Issue 40, 2018 

10. Mukesh Kumar Kumawat, Mukeshchand Thakur, Raju B Gurung, Rohit Srivastava. Graphene Quantum Dots from Mangifera indica: Application in Near-Infrared Bioimaging and Intracellular Nanothermometry. ACS Sustainable Chemistry & Engineering 5 (2), 1382-1391 

11. M Thakur, MK Kumawat, R Srivastava. Multifunctional graphene quantum dots for combined photothermal and photodynamic therapy coupled with cancer cell tracking applications. RSC Advances 7 (9), 5251-5261 

12. MK Kumawat, M Thakur, RB Gurung, R Srivastava. Graphene quantum dots for cell proliferation, nucleus imaging, and photoluminescent sensing applications. Scientific reports 7 (1), 15858

13. MK Kumawat, M Thakur, JR Lakkakula, D Divakaran, R Srivastava. Evolution of thiol-capped gold nanoclusters into larger gold nanoparticles under electron beam irradiation. Micron 95, 1-6 

14. Subhadeep Das, Mukesh K Kumawat, Srivastav Ranganathan, Rakesh Kumar, Jozef Adamcik, Pradeep Kadu, Ranjith Padinhateeri, Rohit Srivastava, Raffaele. Mezzenga, Samir. Maji. Cell Alignment on Graphene–Amyloid Composites. Advanced Materials Interfaces 1800621 

15. JR Lakkakula, D Divakaran, M Thakur, MK Kumawat, R Srivastava. Cyclodextrin-stabilized Gold nanoclusters for bioimaging and selective labelfree intracellular sensing of Co2+ ions. Sensors and Actuators B: Chemical 262, 270-281 

16. Krista R Khiangte,JS Rathore, S Das, RS Pokharia, J Schmidt, H J Osten, S. Mahapatra et al J Appl Phys 124,065704(2018)

 

17. Krista R Khiangte,J S Rathore, J Schmidt, H J Osten, A LahaS. Mahapatra et al Applied Phys 51, 32(2018)

18. “Recovery of Active Surface Sites of Shape-Controlled Platinum Nanoparticles Contaminated with Halide Ions and Its Effect on Surface-Structure”, Ruttala Devivaraprasad, Tathagata Kar, Pradipkumar Leuaa, and Manoj Neergat, Journal of The Electrochemical Society, 164 (2017), H551 H560

 2021: 

1. Jinal M. Mehta, Nishant K. Jain, Deepak S. Chauhan, Rajendra Prasad, Mukesh K. Kumawat, Mukesh Dhanka, Asifkhan Shanavas, Rohit Srivastava, Emissive radiodense stealth plasmonic nanohybrid as X-ray contrast and photo-ablative agent of cancer cells, Materials Today Communications, Volume 27, 2021,https://doi.org/10.1016/ j.mtcomm.2021.102181 

2. Barkha Singh, Rohan Bahadur, Misah Rangara, Mayuri N. Gandhi, and Rohit Srivastava*, Influence of Surface States on the Optical and Cellular Property of Thermally Stable Red Emissive Graphitic Carbon Dots, ACS Appl. Bio Mater.2021, 4, 5, 4641–4651 

3. Anivind Kaur Bindra, Sivaramapanicker Sreejith, Rajendra Prasad, Mahadeo Gorain, Rijil Thomas, Deblin Jana, Mui HoonNai, Dongdong Wang, Abhimanyu https://ams.iitb.ac.in/d/58350-XQQMS9BXXYQGX5C3 Tharayil, Gopal C. Kundu, Rohit Srivastava, Sabu Thomas, Chwee Teck Lim, Yanli Zhao, A Plasmonic Supramolecular Nanohybrid as a Contrast Agent for Site- Selective Computed Tomography Imaging of Tumor 

4. Das, S.; Rakshit, S.; Datta, A. Mechanistic Insights into Selective Sensing of Pb2+ in Water by Photoluminescent CdS Quantum Dots. J. Phys. Chem. C 2021, 125 (28), 15396–1540 

 

 

5. Ali, F.; Das, S.; Banerjee, S.; Maddala, B. G.; Rana, G.; Datta, A. Intense Photoluminescence from Cu-Doped CdSe Nanotetrapods Triggered by Ultrafast Hole Capture. Nanoscale 2021, 13 (33), 14228–14235 

6. Gogoi, H.; Maddala, B. G.; Ali, F.; Datta, A. Role of Solvent in Electron-Phonon Relaxation Dynamics in Core-Shell Au−SiO2 Nanoparticles. Chem Phys Chem 2021, 22 (21), 2201–2206 

7. Shally Sharma, Biomass-Derived Activated Carbon-Supported Copper Catalyst: An Efficient Heterogeneous Magnetic Catalyst for Base-Free Chan−Lam Coupling and Oxidations, ACS Omega, 6, 2021, Page no. 19529-19545 

8. Prateek Bhardwaj, Jayant Sastri Goda, Venkatesh Pai, Pradip Chaudhari, Bhabani Mohanty, Trupti Pai, Komal Vishwakarma, Rahul Thorat, Tabassum Wadasadawala, Rinti Banerjee, Ultrasound augments on-demand breast tumor radiosensitization and apoptosis through a tri-responsive combinatorial delivery theranostic platform, Nanoscale, 13 (2021), 17077-17092 

9. Bera, A.; Pathak, S. S.; Kotha, V.; Prasad, B. L. V. Lamellar Bimetallic Thiolates: Synthesis, Characterization, and Their Utilization for the Preparation of Bimetallic Chalcogenide Nanocrystals through Mechanochemical Grinding. Adv. Mater. Interfaces 2021, 8 (23), 2100898

 10. Maurya, S. K.; Swaroop Pathak, S.; Panchakarla, L. S.; Singh, H. B. Synthesis and Self-Assembly of Amphiphilic Ferrocene-Selenopeptide Conjugates. European J. Org. Chem. 2022, 2022 (6), e202101363 

11. Pathak, S. S.; Panchakarla, L. S. A Large Area Flexible P-Type Transparent Conducting CuS Ultrathin Films Generated at Liquid-Liquid Interface. Appl. Mater. Today 2021, 24, 101152

 

2022:

 1. Prateek Bhardwaj, Vikram Gota, Komal Vishwakarma, Venkatesh Pai, Pradip Chaudhari, Bhabani Mohanty, Rahul Thorat, Subhash Yadav, Murari Gurjar, Jayant Sastri Goda, Rinti Banerjee, Loco-regional radiosensitizing nanoparticles-in-gel augments head and neck cancer chemoradiotherapy, Journal of Controlled Release, 343 (2022), 288-302.

 2. Radhika Poojari, Bhabani Mohanty, Vijay Kadwad, Dayaram Suryawanshi, Pradip Chaudhari, Bharat Khade, Rohit Srivastava, Sanjay Gupta, Dulal Panda, Combinatorial cetuximab targeted polymeric nanocomplexes reduce PRC1 level and abrogate growth of metastatic hepatocellular carcinoma in vivo with efficient radionuclide uptake, Nanomedicine: Nanotechnology, Biology and Medicine, Volume 41, 2022, 102529, https://doi.org/10.1016/j.nano.2022.102529.

 3. Pranjali Yadav, Shubhra Chaturvedi, Samir Kumar Biswas, Rohit Srivastava, KamalakannanKailasam*, Anil Kumar Mishra*, and AsifkhanShanavas*,Biodegradable Protein-Stabilized Inorganic Nanoassemblies for Photothermal Radiotherapy of Hepatoma Cells, , ACS Omega 2022 

4. Single particle level dynamics of photoactivation and suppression of Auger recombination in aqueous Cu-doped CdS quantum dots, Sharmistha Das, Gourab Rana , Fariyad Ali and Anindya Datta, Nanoscale, 2023,15, 4469-4476 

5. Carbon nanodot doped in polymer film: Plasmophore enhancement, catalytic amination and white-light generation, Farogh Abbas, Sagar Kumar, Surja Kanta Pal, Debashis Panda, Journal of Molecular Liquids, 2022, 347,118001 

6. Enhancement of Antimycobacterial Activity of Rifampicin Using Mannose-Anchored Lipid Nanoparticles against Intramacrophage Mycobacteria Nishita Mistry, Rajdip Bandyopadhyaya, and Sarika Mehra, ACS Appl. Bio Mater. 2022, 5, 5779−5789

 

7. Yb3+-Doped Phenylethyl ammonium Lead Bromide 2D Layered Hybrid Perovskite for Near- Infrared Emission, Barnali Mondal, Athunya Poovathan, Dr. Tariq Sheikh, Prof. Angshuman Nag ChemNanoMat, 2022, 8, e202200104 

8. Non-aqueous rechargeable calcium-ion batteries based on high voltage zirconium-doped ammonium vanadium oxide cathode Author links open overlay panelMd Adil, Ananta Sarkar, Supriya Sau, Divyamahalakshmi Muthuraj, Sagar Mitra Journal of Power Sources 2022, 541,231669

9. Carbon coated titanium dioxide (CC-TiO2) as an efficient material for photocatalytic degradation. Rahul Kumar, Raveena Choudhary, Santa Kolay, O. P. Pandey, Kulvir Singh and Parag Bhargava Energy Adv., 2022, 1, 926-934 

10. Comprehensive Study of Sodium Copper Hexacyanoferrate, as a Sodium-Rich Low-Cost Positive Electrode for Sodium-Ion Batteries Md. Adil, Supriya Sau, Pradeep Dammala, and Sagar Mitra, Energy Fuels 2022, 36, 7816−7828 

11. In vivo efficacy & phantom imaging connote the theranostic potential of a drug-loaded lipid nanobubble. Authors Ekta Singh, Rinti Banerjee Journal of Drug Delivery Science and Technology 2022, 74,103568

12. Water-in-Salt Electrolyte-Based Extended Voltage Range, Safe, and Long-Cycle-Life Aqueous Calcium-Ion Cells Md. Adil, Arpita Ghosh, and Sagar Mitra, ACS Appl. Mater.Interfaces 2022, 14, 25501−25515 

13. Sooting propensities of novel cage hydrocarbon propellants, Anand Sankaranarayanan, Nitesh Gupta, Sohan Lal Irishi N Namboothiri , Arindrajit Chowdhury , Neeraj Kumbhakarna, Fuel 2022, 329, 125437

14. Effect of dispersant added graphene nanoplatelets with diesel–Sterculia foetida seed oil biodiesel blends on diesel engine: engine combustion, performance and exhaust emissions, Gandhi Pullagura Srinivas Vadapalli ,Prasad V. V. S.& Kodanda Rama Rao

Chebattina, BioFuels 2022, 14, 2148876

15. Na2ZrFe(PO4)3 A Rhombohedral NASICON-Structured Material: Synthesis, Structure and Na Intercalation Behaviorc, Anil K. Paidi, Ankur Sharma, Vinod K. Paidi, Mani Pujitha Illa, Kug-Seung Lee, Sangsul Lee, Docheon Ahn, and Amartya Mukhopadhyay , Inorg. Chem.2023, 62, 4124−4135 

16. Combinatorial cetuximab targeted polymeric nanocomplexes reduce PRC1 level and abrogate growth of metastatic hepatocellular carcinoma in vivo with efficient radionuclide uptake,Radhika Poojari, Bhabani Mohanty , Vijay Kadwad , Dayaram Suryawanshi ,Pradip Chaudhari , Bharat Khade , Rohit Srivastava , Sanjay Gupta , Dulal Panda PhD Nanomedicine: Nanotechnology, Biology and Medicine 2022, 41, 102529

17. Influence of Al3+-Gd3+ co-substitution on the structural, morphological, magnetic and optical properties of nickel ferrite nanoparticles Sanjay B. Gopale, Mangesh V. Khedkar, Swapnil A. Jadhav, Anil V. Raut, Sunil S. Karad, Govind D. Kulkarni & K. M. Jadhav, Journal of Materials Science: Materials in Electronics 2022, 33, 26544–26563 

18. Kinetic and Mechanistic Insight into the Surfactant-Induced Aggregation of Gold Nanoparticles and Their Catalytic Efficacy: Importance of Surface Restructuring Published as part of The Journal of Physical Chemistry virtual special issue “Kankan Bhattacharyya Festschrift”. Bhawna Saini, Laxmikanta Khamari, and Tushar Kanti Mukherjee, J. Phys.Chem. B 2022, 126, 2130−2141

19. α-Synuclein Aggregation Intermediates form Fibril Polymorphs with Distinct Prion-like Propertiespanel,Surabhi Mehra , Sahil Ahlawat ,Harish Kumar ,Debalina Datta , Ambuja N avalkar , Nitu Singh , Komal Patel , Laxmikant Gadhe , Pradeep Kadu , Rakesh Kumar , Narendra N. Jha, Arunima Sakunthala , Ajay S. Sawner , Ranjith Padinhateeri , Jayant B. Udgaonkar , Vipin Agarwal , Samir K. Maji, Journal of molecular Biology 2022 434, 167761

 

20. Synthetic Protocell as Efficient Bioreactor: Enzymatic Superactivity and Ultrasensitive Glucose Sensing in Urine, Bhawna Saini and Tushar Kanti Mukherjee, ACS Appl. Mater. Interfaces 2022, 14, 53462−53474

21. An Ultrasound-Responsive Theranostic Cyclodextrin-Loaded Nanoparticle for Multimodal Imaging and Therapy for Atherosclerosis. Sourabh Mehta, Viktoria Bongcaron, Tien K Nguyen, Yugandhara Jirwanka, Ana Maluenda, Aidan P G Walsh, Jathushan Palasubramaniam, Mark D Hulett, Rohit Srivastava, Alex Bobik, Xiaowei Wang, Karlheinz Peter Small, 2022, 18, 202200967.

2023: 

1. Single particle level dynamics of photoactivation and suppression of Auger recombination in aqueous Cu-doped CdS quantum dots, Sharmistha Das, Gourab Rana , Fariyad Ali and Anindya Datta, Nanoscale, 2023,15, 4469-4476