External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
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Category
- Microscopy and Imaging » Optical Microscopy
- Spectroscopy and Spectrometry » Optical Spectroscopy
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Typhoon FLA 9500 is a robust and versatile laser scanner that is used of biomolecular imaging applications like western blots, multiples fluorescence, and radioisotopic labels by storage phosphor as well as digitization of colorimetric stains.
Detection mode: Fluorescence, Phosphorimaging, digitization and Chemiluminescence
Excitation Wavelengths:473 nm (blue LD) laser, 532 nm (green SHG laser), 635 nm (red LD laser), 685 nm (near IR LD laser), 785 nm (near IR LD laser)
Bit Depth: 16-bit
Standard Filters: IP (Phosphorimaging), LPB (510LP), LPG (575LP), LPR (665LP), LPR-Ch2 (665LP), BPB1 (530DF20) AND BPG1 (570DF20/665LP)
Radioisotopes:3H, 11C, 14C, 125I, 18F, 32P, 33P, 35S, 99mTc
Sample Preparation, User Instructions and Precautionary Measures
Typhoon FLA 9500 Biomolecular Imager system charges excluding 18% GST
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Charges for Analytical Services in Different Categories
Applications
Typhoon FLA 9500 is a versatile laser scanner for biomolecular imaging applications, including the following:
Sensitive and quantitative measurement of radioisotopic labels
2D DIGE
Visible single channel and multiplex fluorescence (ECL Plex Western blotting systems
Near infrared fluorescence (optional)
Colorimetric stains (e.g., Coomassie blue and silver-stained gels)
Imaging of gel, membrane, etc. dyed or labelled with various fluorescent dyes at high sensitivity and high resolution
Features very high sensitivity, wide dynamic range, high linearity, and high resolution
Reusable storage phosphor screens