External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
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Category
- Chromatography » Liquid Chromatography
- Spectroscopy and Spectrometry » Mass Spectrometry
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Working Principle
Liquid chromatography / Mass Spectroscopy (LC / MS) is a technique which combines high performance liquid chromatography HPLC, a powerful analytical separation technique with mass spectroscopy ,a powerful analysis & detection technique. There are two common atmospheric pressure ionization (API) LC/MS process: Electrospray Ionization (ESI) & Atmospheric Pressure Chemical Ionization (APCI). Both are soft ionization technique. Agilent iFunnel technology combines highly efficient electrospray ion generation and focusing of Agilent Jet Stream technology with a hexabore capillary sampling array and dual-stage ion funnel for increased ion sampling and transmission. NanoHPLC combined with mass spectrometer can analysed small molecule as well large molecules like proteins UHPLC separations can be detected by PDA & Mass spectrometer as different detectors.
MS/MS function
- Sample Injection
- Ionization
- Fragmentation (CID)
- Ions travel through Quadruple and Time flight tube and reach the detector on the basis of their m/z value.
Specification:
- UHPLC & NanoHPLC for small molecules as well as for large molecules
- Direct Infusion Mass with ESI & APCI (Positive & Negative mode ionization)
- UHPLC PDA Detector - Mass spectrometer
Mass Range : 50-3200 amu
Resolution : 40000 FWHM
High Mass Accuracy (Typically less than 1ppm)
High Sensitivity (1 pg. Reserpine S/N 100:1)
Ionization Method : API
1) ESI positive & negative 2) APCI positive & negative
Direct Infusion for Mass Analysis ( MS , MS /MS)
UHPLC PDA Mass Spectrometer.
Sample Preparation, User Instructions and Precautionary Measures
1. Expected structure or mass can be provided.
2. Solvent for mobile phase should be mentioned or solubility of compound in volatile solvent
3. As HP-LC is available in most departments, LC of submitted samples indicating the operation should be supplied along with the sample.
4. At present C8,C18 & others like cyano, amino, Si columns are available. You can submit the interest for separation or repeatability of HP-LC run.
5. For HPLC PDA analysis, wavelength should be given.
6. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous.
Samples will not be accepted unless MS-DS.
Kindly download Material Safety Data sheet and submit the hardcopy of filled sheet along with your sample.
Charges for Analytical Services in Different Categories
HRLCMS QTOF | |||||
| IITB User | University | National Lab/R&D's; Sine (Letter from SINE required); Research Park (MSME) (Letter from RP required) | Start Up | Industry |
ONLY HRMS | 650 | 1300 | 3250 | 4875 | 6500 |
HRMS+COPMPOUND FORMULA | 865 | 1725 | 4325 | 6488 | 8650 |
LCMS with library (Plant extract Impurity Profiling Metabolite Identification or Degradation studies or Environmental samples) | 1450 | 2900 | 7250 | 10875 | 14500 |
Amino acid sample preparation | 650 | 1300 | 3250 | 4875 | 6500 |
HPLC with PDA detector for amino acid profiling | 805 | 1610 | 4025 | 6038 | 8050 |
HPLC + PDA detector + HRMS Spectrometer with library | 1600 | 3220 | 8050 | 12000 | 16000 |
HPLC with PDA detector | 805 | 1610 | 4025 | 6038 | 8050 |
Applications
Qualitative Analysis & Quantitative Analysis
Impurity Profiling
Metabolite Identification
Pharmacokinetics
Pharma & biomedical applications
Amino acid profiling of 22 amino acid
Degradation studies
Environmental samples