Specifications: 

• Vanquish UHPLC for small molecules & Nano-HPLC for large molecules
• Direct Infusion Mass with ESI & APCI (Positive & Negative mode ionization)
• Quaternary UHPLC with Variable Wavelength Detector Mass Spectrometer
• Nano HPLC (Micro fluidic column) - Mass spectrometer

Unique Features of the Q-Exactive Plus: 

• Characterize, quantify and confirm with unmatched confidence using the Q-Exactive Plus mass spectrometer.
• Advanced Quadrupole technology featuring Thermo Fisher segmented HyperQuad.
• Resolving power up to 280,000.
• Maximum scan speed 12 Hz.
• Spectral multiplexing for enhanced duty cycle.
• RF-Lens ion source for increased sensitivity.
• Advanced Active Beam Guide intelligent ion beam management for high flux ion sources.
• Intact Protein Mode option for characterizing intact proteins with ease.
• Enhanced Resolution Option maximizing resolution at 280,000.

Working Principle 

The Orbitrap is an ion trap mass analyzer consisting of two outer electrodes and a central electrode, which enable it to act as both an analyzer and detector. Ions entering the Orbitrap are captured through "electrodynamic squeezing." Once captured, ions oscillate around the central electrode and between the two outer electrodes at different frequencies, depending on their mass-to-charge ratios. This oscillation separates the ions. By measuring these oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection.

Liquid Chromatography / Mass Spectrometry (LC/MS) combines high-performance liquid chromatography (HPLC) for separation with mass spectrometry for analysis and detection. It utilizes two common atmospheric pressure ionization (API) processes: Electrospray Ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI), both of which are soft ionization techniques.

The Q Exactive Plus mass spectrometer provides fast, sensitive, reliable, and confident detection and identification of compounds in complex mixtures while maintaining full compatibility with UHPLC and fast chromatography. The HCD collision cell enhances functionality by providing ion fragmentation while preserving high resolution, accurate mass, and high sensitivity.

Main Features of Instrument 

• Mass Range (m/z): 50-8000 amu
• Resolution: 140,000 @ m/z 200 (280,000 with Enhanced Resolution mode option)
• High Mass Accuracy (Typically less than 1 ppm)
• High Sensitivity (500 fg of Buspirone, S/N 100:1)
• Ionization Method:
  • API:
    1. HESI positive & negative
    2. APCI positive & negative
• Direct Infusion for Mass Analysis (MS, MS/MS)
• Binary Nano HP-LC system with Mass as detector
• Quaternary UHPLC with Variable Wavelength Detector Mass Spectrometer