Facility Name
External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”
Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.
Make
BSL-2 Facility Tissue Culture Rooms
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)-IoE Funded
.
Category
- Services » Service
Booking Details
Booking available for
Internal and External Both
Available Equipment/ Mode of use
Electronic Weight Balance (Model BT 224S)
Rotary Vacuum Evaporator with heating bath and associated controller, vapour temperature sensor, vacuum trap and vacuum pump (Model No. 8703 RV.0.000
HETTICH, Germany Table top Refrigerated Centrifuge, Model: Universal 320 R etc… Cat. No. 1406
Vertical Down Flow Work Station of S.S. 304 Construction; Standard Feature: Model No. 4x2, Hepa Filter Size: 48 x 24 x4; Overall Dimensions: 51" x 30" x 81"; Filter, Pre-Filter; Motor Blower; Manometers, Air Cleanliness; Qty 2
Part No. 1420-040: Victor V Microplate Reader for 230V/50 Hz operation; It includes 1. Fluorescence (Top & Bottom) (340-850 nm); 2. Luminescence (Flash, Glow & Duel); 3. Visible Absorbance (340 nm - 800 nm); 4. UV Absorbance (260nm / 280 nm); 5. Time Reso
Equitron Laboratory Autoclave the New Single Lever Lock Model SLL - REGULAR SERIES
CRYOCAN MODEL BA-20 WITH CANISTERS
CRYOCAN MODEL BA-35 WITH CANISTERS
"1. HETTICH, Germany Table top Microlitre Centrifuge, Model: MIKRO 120, 230V, 50Hz. Capacity: 24 x 1.5 / 2.0 ml. Speed: 14000 rpm/17,530 RCF. Impulse key, Electronic display, RPM/RCF. Temperature Catalog No. 1204. 2. Angle Rotor, 24-place, 2-row for 1.5/
SANYO, Professional Cell Culture CO2 Incubator, Make airjacketed, 230V 50 Hz Model: MCO-15AC
SANYO -86°C Deep Freezer, Upright Model with Patented VIP Technology, 230V. 50 Hz; Model: MDF-U32V; Ultra Low Temperature Freezer
Olympus Microscope with 10X, 40X and 60X objectives; Fluorescence Microscope with Camera and Computer
Double Door Fridge
4°C Fridge
Facility Management Team and Location
Facility In Charge
Prof. Shobhna Kapoor
Co-convenors
Prof. Shobhna Kapoor
Facility Management Members
Prof. Venkateswarlu Yarlagadda
Prof. Rajdip Bandyopadhyaya
Prof. Anindya Datta
Prof. Shobhna Kapoor
Department
Centre for Research in Nanotechnology and Science (CRNTS)
Lab Email ID
tissueculture208b@gmail.com
Facility Location
Room No. 208B, First Floor, CRNTS-SAIF Building, IIT Bombay, Powai, Mumbai 400076
Lab Phone No
NA
Facility Features, Working Principle and Specifications
Facility Description
Facility Description
This facility is used for culturing mammalian cells pertaining to BSL-1 and BSL-2 conditions.
Features Working Principle
The Tissue Culture Facility is a biosafety-compliant laboratory designed for the culture, maintenance, and experimentation on mammalian samples under Biosafety Level 1 (BSL-1) and Biosafety Level 2 (BSL-2) containment. The facility is equipped to support a wide range of cell biology, microbiology research while ensuring operator safety, sterility, and environmental containment.
- HEPA-filtered ventilation system ensuring proper airflow and containment.
Biosafety Cabinets for aseptic handling of biological samples - CO₂ Incubators – maintaining precise temperature, humidity, and CO₂ levels for mammalian cell culture.
- Phase-Contrast and fluorescence microscope – for real-time cell monitoring.
- Refrigerators & Freezers (4°C, -10-15°C, -80°C) – for media, reagents, and sample storage.
- Liquid Nitrogen Cryostorage System – for long-term cell line preservation.
- Centrifuges (Tabletop & High-speed) – for sample preparation and cell pelleting.
- Autoclave
Rules:
- Use of non-pathogenic cell lines.
- Basic aseptic techniques for contamination control.
- General waste disposal following standard guidelines.
- Proper disinfection protocols (70% ethanol, bleach, UV sterilization).
This facility provides a state-of-the-art, safe, and controlled environment for academic and industrial research in cell biology, infectious diseases, and biomedical sciences.
Sample Preparation, User Instructions and Precautionary Measures
User Instructions and Precautionary Measures
- Access Control – Entry is restricted to authorized personnel only.
- Personal Protective Equipment (PPE) – Always wear a lab coat, gloves and shower caps.
- Aseptic Handling – Work inside the Class II Biosafety Cabinet for all cell culture procedures.
- Equipment Use – Follow SOPs for incubators, centrifuges, microscopes, and biosafety cabinets. Clean equipment after use.
- Waste Disposal – Dispose of biohazardous waste in designated bins. Chemically disinfect before disposal.
- Labeling – Clearly label all cultures, reagents, and waste containers with date and contents.
- Spill Response – In case of a spill, immediately contain, disinfect, and report.
- Precautionary Measures:
No food, drinks, or personal items inside the lab.
Always work with BSL-2 agents under containment in a biosafety cabinet.
Decontaminate work surfaces before and after use (70% ethanol).
Avoid aerosol generation when pipetting or centrifuging samples.
Use mechanical pipetting devices – never pipette by mouth.
Handle sharp objects with care and dispose of them in designated sharps containers.
Report any accidents, exposures, or equipment malfunctions immediately.
Following these guidelines ensures safety, sterility, and compliance while working in the tissue culture facility.
Charges for Analytical Services in Different Categories
Usage Charges
| Description | Academic | National R & D Lab | Industry/Non Govt. Agencies |
| Per 2 Hrs slot | ₹ 2500/- | ₹ 2500/- | ₹ 4000/- |