Make
BRUKER. Switzerland
Model
AVANCE III HD
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)
.
Category
- Spectroscopy and Spectrometry » NMR Spectroscopy
Booking Details
Slot Booking Link
Booking available for
Internal and External Both
Available Equipment/ Mode of use
he 400 MHz Bruker AVANCE NEO NMR Spectrometer is a versatile, high-performance system designed to support a wide range of research applications.
Facility Management Team and Location
Facility In Charge
Prof. Chandra M.R. Volla
Prof. Arnab Datta
Facility Manager
Mr. Niteen Kadam
Mr. Narendra Gaikwad
Facility Operator
Mr. Vaibhav Nagare
Facility Management Members
Prof. Santosh Gharpure
Prof. Sanjog Nagarkar
Prof. Ishita Sengupta
Prof. Venkateswarlu Yarlagadda
Prof. Prbhakar Naraga
Prof. Vikram Vishal
Department
Chemistry
Lab Email ID
niteen_kadam@iitb.ac.in
Facility Location
Room No. CL-147, Gr. Floor, Chemistry Department I.I.T. Bombay, Powai, Mumbai - 400076
Lab Phone No
02225764154
Facility Features, Working Principle and Specifications
Facility Description
Facility Description
This facility provides advanced capabilities for the analysis of liquid samples and real-time reaction monitoring.
Advanced Probes
- 5 mm Multinuclear i-Probe: Designed for liquid-state studies of nuclei such as ^1H, ^13C, ^19F, ^31P, ^11B, ^27Al, ^77Se, ^125Te, ^199Hg, and more.
- 5 mm Double Resonance ^13C/^1H Probe: Optimized for experiments requiring simultaneous detection of ^1H and ^13C nuclei.
Experiment Capabilities
- 1D NMR: Includes experiments like ^1H, ^13C, NOE, APT, DEPT, and more.
- 2D NMR: Supports advanced techniques such as COSY, NOESY, TOCSY, HSQC, HMBC, DOSY, and ROESY.
- Variable Temperature (VT): Enables experiments in the temperature range of -40°C to +100°C.
Sample Types
- Liquid Samples: Requires samples to be soluble in 0.6 mL of a suitable solvent.
- Real-Time Reaction Monitoring: Enables kinetic studies and process optimization with in situ monitoring.
- 24-Sample Auto-Sampler: Facilitates high-throughput analysis by automating the handling of up to 24 samples.
Features Working Principle
The spectrometer uses Fourier Transform Nuclear Magnetic Resonance (FT-NMR) to provide high-resolution spectra, offering insights into molecular structure, dynamics, and interactions. Real-time reaction monitoring integrates seamlessly, enabling simultaneous acquisition of kinetic data and structural information.
Body Specification
The system features robust hardware for long-term stability, advanced cryogenics for temperature regulation, and state-of-the-art software for data acquisition and analysis.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
Instructions for Registration
1: Submit an Official Request Letter
- The letter must be on your institution's official letterhead.
- It should be signed by the Head of Department (HoD) or Research Guide.
- The letter must confirm that the analysis is for research purposes (to qualify for academic concessions).
2: Address the Letter To
The Head, Department of Chemistry, IIT Bombay.
3: Include the Following Details in the Letter
- Type of Solution NMR Analysis Required
- Mention the nuclei for analysis (e.g., ¹H, ¹³C, ³¹P, ¹⁹F, etc.).
- Type of Experiment Required
- Specify the required experiment (e.g., 1D NMR: ¹H, ¹³C, DEPT, APT, or 2D NMR: COSY, NOESY, HSQC, HMBC, DOSY, etc.).
- Sample Details
- Provide details such as:
- Solvent Used (e.g., CDCl₃, D₂O, DMSO-d₆, etc.)
- Sample Concentration
- Any Special Handling Requirements
- industrial research needs.
- Provide details such as:
Instruction for Sample Preparation
- Ensure complete solubility in 0.6 mL of the selected solvent.
- Required amounts:
- 1H NMR: 10-20 mg (depending on molecular weight).
- 13C NMR: 20-30 mg for adequate signal-to-noise ratio.
User Instructions and Precautionary Measures
BBefore Using the Facility:
- Read and understand the instrument manual and SOPs.
- Prepare the sample in a deuterated solvent (e.g., CDCl₃, D₂O, DMSO-d₆) to ensure proper locking and shimming.
- Filter the sample, if necessary, to remove insoluble particles that could interfere with the experiment.
- Use a clean and undamaged NMR tube with the correct length and thickness.
During Experiments:
- Handle NMR tubes carefully to prevent breakage.
- Insert the NMR tube gently into the spinner to avoid misalignment.
- Do not exceed the recommended sample volume (typically 0.6 mL for standard 5 mm tubes).
- Ensure proper shimming and tuning before starting data acquisition.
After Using the Facility:
- Remove the sample from the spectrometer and clean any spills.
- Collect your NMR spectra and save the data for future reference.
- Retrieve your unused sample material and dispose of waste properly.
Report any instrument issues or irregularities to the lab personnel.
Important Note for External Users:
- External users are NOT allowed to operate the instrument.
- Only authorized facility personnel will handle the sample preparation, experiment setup, and data acquisition for external users.
- External users must submit their samples along with a properly filled analysis request form and the required payment (if applicable).
- Users will receive the processed data once the analysis is complete.
Charges for Analytical Services in Different Categories
Usage Charges
400 MHz NMR II (CHARGES)
Expt. type | Internal | University (X) | National Labs (3X) | Industries (5X) | |
| Chemistry Dept. | Other IITB Departments (X) |
|
|
|
1H | 50 | 100 | 100 | 300 | 500 |
X-nuclei | 50 | 200 | 200 | 600 | 1000 |
Subsequent hour | 50 | 150 | 150 | 450 | 750 |
2D NMR | 50 | 300 | 300 | 900 | 1500 |
Additional Charges for Variable temp. (Solution/Solid) | 50 | 250 | 250 | 750 | 1250 |
Applications
- Major application of NMR spectroscopy lies in the area of synthetic organic chemistry, inorganic chemistry, bio-organic chemistry, bio-inorganic chemistry, polymer chemistry and organometallic chemistry. One of the main advantages of FT-NMR spectroscopy is that a number of FIDs can be accumulated and Fourier transformed, enhancing S/N ratio of the spectrum. This in turn facilitates the analysis of samples with low abundant nuclei and smaller gyro-magnetic ratios, e.g.,13C, 29Si, 15N etc.
- NMR studies find applications in some of following areas :
- Molecular conformation in solution
- Quantitative analysis of mixtures containing known compounds
- Determining the content and purity of a sample
- Through space connectivity (Overhauser effect)
Sample Details
Chemical allowed
NA
Allowed Substrate
NA
Gases allowed
NA
Substrate Dimension
NA
Target dimension
NA
Contamination remarks
NA
Precursors/ Targets allowed
NA