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Category
- Spectroscopy and Spectrometry » Mass Spectrometry
Booking Details
Facility Management Team and Location
Email: - amritan@iitb.ac.in
Phone: +91-22-25767858
Email: - amritan@iitb.ac.in
Phone: +91-22-25767858
Email: - mitras@iitb.ac.in
Phone: +91-22-25767854
Email: - wangikar@iitb.ac.in
Phone: +91-22-25767232
Email: - ishita@chem.iitb.ac.in
Phone: +91-22-25767167
Facility Features, Working Principle and Specifications
Facility Description
The UHPLC-HRMS Orbitrap (Ultra-High-Performance Liquid Chromatography coupled with High-Resolution Mass Spectrometry using an Orbitrap analyzer) is an advanced analytical instrument widely used for the precise identification of chemical compounds. It combines the high separation efficiency of UHPLC with the exceptional mass accuracy and resolution of the Orbitrap mass analyzer.
Sample Injection & Separation:
The liquid sample is introduced into the UHPLC system, where it undergoes rapid and efficient chromatographic separation into individual components.
Ionization:
The separated components are converted into charged ions by ionization techniques like HESI or APCI.
Ion Transmission to Orbitrap:
The ions are guided into the Orbitrap mass analyzer after initial filtration or focusing using electric and magnetic fields
Mass Analysis in Orbitrap:
Ions entering the Orbitrap are captured through "electrodynamic squeezing," after which they oscillate around the central electrode and in between the two outer electrodes. Different ions oscillate at different frequencies, resulting in their separation. By measuring the oscillation frequencies induced by ions on the outer electrodes, the mass spectra of the ions are acquired using image current detection.
Data Analysis:
The data is processed using software for identifying molecular weights, isotopic patterns, and fragmentation data, enabling compound identification, and structural elucidation.
Resolution: 70,000 FWHM
Mass range:50 to 2000 amu
Ionization method: HESI/APCI
High mass accuracy (in the range of ppb)
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
An appointment will be given as per queue and will be informed by email.
At least 24 hours prior information should be given to ESED lab if users want to cancel/postpone the slot.
Sample preparation must be completed by the user.
The sample must be filtered using a 0.22 µm nylon filter.
Ensure that analyte concentrations are less than 3 ppm
1. First discuss with the facility personal about the nature of sample and required analysis. Please proceed with payment only when you get a confirmation that the required analysis is feasible.
2. A requisition letter addressed to Head, ESED along with a printout of electronic fund transfer (NEFT) proof should be sent by post or submitted in person to ESED office, IIT Bombay, Powai, Mumbai-400076.
3. If you are sending samples by post please complete al details in Requisition form.
4. Once we receive the requisition form and advance payment, your appointment will be scheduled as per the queue.
5. The users will be informed about their date and time of slot by e-mail.
6. We prefer that you or your representative, who knows/understands the sample/material should be present on the day of appointment.
7. Before proceeding further, please review How to Use Facility and Payment Procedure for more information.
Charges for Analytical Services in Different Categories
Applications
Applications:
- Qualitative analysis of compounds
- Environmental sample analysis
- Pharma and biomedical sample analysis
- Degradation studies
- Degradation pathway analysis
Sample Details
Non-Toxic/Harmful, Non-Infectious/Harmful, Non-Explosive
Non-Toxic/Harmful, Non-Infectious/Harmful, Non-Explosive
Vial Size: - 2ml
Non Harmful Chemically/ Biologically, Non-Explosive