Matrix assisted laser desorption/ ionisation- time of flight facility
Matrix-assisted laser desorption/ionization is a soft ionization technique used in mass spectrometry, allowing the analysis of biomolecules (biopolymers such as DNA, proteins, peptides and sugars) and large organic molecules (such as polymers and other macromolecules). It is used for the study of protein/peptide characterization, polymer analysis, glycan analysis, nucleic acids & small molecule analysis.
Make
Bruker
Model
Autoflex Speed
Facility Status
Working
Date of Installation
Facility Management Division
Institute Central Research Facilities (ICRF)

Category

  • Spectroscopy and Spectrometry » Mass Spectrometry

Booking Details

Slot Booking Link
Click here for Booking!
Booking available for
Internal and External Both
Available Mode for Use
Linear mode- For high molecular weight analytes (>10,000Da) Reflectron mode- For low molecular weight analytes (<10,000Da)

Facility Management Team and Location

Faculty In Charge
Prof. Ashutosh Kumar, ashutoshk@iitb.ac.in, +(91-22) 2576 7762
Facility Operator
Nitin Bhoite, 4757
Co-convenors
Prof.Sanjeeva Srivastava
Prof. Samir K. Maji
Prof. NP S Phale
Prof. Pradeep Kumar P I
Prof. Suvarn S. Kulkarni
Prof. Maheswaran S
Prof. Kamendra P. Sharma
Prof. Sarika Mehra
Department
Biosciences and Bioengineering
LAB Email ID
maldi.bios@iitb.ac.in
Facility Location
Room No- 307, Central Instrumentation Facility, Department of Biosciences and Bioengineering
Lab Phone No
022 25764757

Facility Features, Working Principle and Specifications

Features Working Principle
  • Different biomolecules can be analyzed
  • High resolution reflectron mode and the latest TOF/TOF technology (LIFT™)
  • Broadband mass resolving power up to 26,000

Matrix-assisted laser desorption/ionization (MALDI) is an innovative technology designed and optimized to be easy-to-use for reliable and detailed protein/peptide characterization, polymer analysis, Glycan analysis, nucleic acids & small molecule analysis.

Role of the matrix :

The matrix plays an important role as it transfers the energy needed for ionisation from the laser light to the sample molecules.

The ionization of the molecules with laser bombardment leads to movement of the ions in vacuum and detected by the detector. Based on the detection of the ionised samples by the detector data acquisition is performed which thereby generates the mass spectra with the help of Auto control & Auto flex softwares.

Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges

Instructions for Registration

For Internal Users (IIT Bombay):

  • Only online sample registration through the IRCC webpage will be accepted.
  • The users will be informed about their date and time of the slot by email.
  • If the user cannot come even after accepting the date & time of the slot, a mail should be immediately sent to maldi.bios@iitb.ac.in to cancel the slot.
  • For re-allocating the slot after cancellation, fresh online registration to be done.
  • Kindly refer the sample preparation instructions and do's & don'ts prior to filling up the online sample request form.
  • Please come prepared with some literature reference papers for the sample to be analysed by MALDI TOF/TOF.
  • After sample submission, the user need not stay back in the MALDI facility and the results will be sent through email.
  • For samples other than Proteins, Peptides, Glycans, Nucleic acids & Polymers, kindly discuss with the concerned person at MALDI facility at 022 25764757, prior to online registration.

Applications

  • Molecular weight determination of intact proteins
  • Partial protein sequencing
  • Protein identification
  • N-terminal sequencing
  • Small molecule detection
  • Nucleic acid and oligo nucleotide detection
  • Glycan detection
  • Polymer detection

Sample Details

SOP, Lab Policies and Other Details

Lab Workshop Presentation
MALDI TOF TOF Workshop.pptx (725.04 KB)

Publications

Publications
NA