Bio-safety level 2 facility
Bio-safety level 2 facility
Make
Klenzaids Contamination Control Pvt. Ltd
Facility Status
Working
Date of Installation
Facility Management Division
Institute Central Research Facilities (ICRF)

Category

  • Services ยป Service

Booking Details

Booking available for
Internal and External Both
Available Mode for Use
For Category-2 pathogens only

Facility Management Team and Location

Faculty In Charge
Prof. Anirban Banerjee, abanerjee@iitb.ac.in, +(91-22) 2576 7794
Facility Manager
Prof. Anirban Banerjee, abanerjee@iitb.ac.in, +(91-22) 2576 7794
Facility Operator
Prof. Anirban Banerjee, abanerjee@iitb.ac.in, +(91-22) 2576 7794
Co-convenors
Prof. Sarika Mehra
Prof. Suparna Mukherji
Prof. Shobhna Kapoor
Prof. Kiran Kondabagil
Prof. Debjani Paul
Department
Biosciences and Bioengineering
LAB Email ID
bsl2@iitb.ac.in
Facility Location
Room No. 201; Biosciences & Bioengineering Department Building

Facility Features, Working Principle and Specifications

Features Working Principle
  • Animal cell culture room
  • Bacterial culture/Infection room
  • Bio-safety cabinets (Type-II A2 and Type-IIB2)
  • Incubators to grow mammalian cells as well as bacteria Centrifuges
  • -80oC freezer to store pathogenic microorganisms
  • Autoclave for bio-hazard disposal

A BSL-2 facility was set up in March, 2015 in Department of Bioscience and Bioengineering by Klenzaids Contamination Control Pvt. Ltd. In 2016, it was converted into a central facility. It is suitable for work involving Risk Group -2 pathogens that pose moderate hazard to personnel and the environment. Examples of agents typically worked with in a BSL-2 include equine encephalitis viruses and HIV, as well as Staphylococcus aureus, Salmonella etc.. It works efficiently to control generation of any aerosols outside the facility by filtering the incoming as well as outgoing air and maintaining a negative pressure. Apart from Bio-safety cabinets, the facility also houses all other necessary equipments to perform experiments which involves pathogen biology, host-pathogen interaction etc. Access to BSL-2 facility is strictly restricted and should be approved by BSL-2 user committee.

Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges

Instructions for Registration
  • External Users can come in-person or send your samples along with a letter from the Head / Guide on your College/Institute /Industry Original Letter Head for registration stating that the analysis is for research purpose to qualify for academic concession. The letter should be addressed to The Head, Dept. Of Chemistry, IIT, Bombay.
  • The analytical data / spectra are provided only for research / development purposes. These can not be used as certificates in legal disputes.
  • Service charges are payable at prevailing rates (Please add 12.36% service tax).
Instruction for Sample Preparation

The facility can only be used for projects that are approved by IBSC. Following IBSC's approval the protocol of different experiments needs to be approved by the user committee. Once the protocols are approved, the user (a PhD student or Post doc) will be trained and made aware of different hazards associated with working inside the facility. Following completion of training, the user will have to sign an undertaking that he/she has been trained and now responsible for any safety related hazards the he/she faces. The user must inform the facility supervisor in case of any accident or spillage at the earliest. If he/she feels sick, they need to inform the doctor about their work in BSL-2 facility and different kind of pathogens that are being handled inside the facility.

User Instructions and Precautionary Measures

Online booking of slots are available through Qreserve software. At a time only 4 people can work inside the facility. A user can book the facility for a maximum of 3 h at a stretch. He/she can book it for another 3 h (depending on the length of the experiment), but with a gap of 1 h in between two consecutive 3 h slots to allow other users to perform minor work in between.

Applications

Pathogen Characteristization

Sample Details

Gases allowed

CO2 Cylinders

Contamination remarks

Sometimes we face several pathogen contaminations

Precursors/ Targets allowed

BSL Category 2 pathogens allowed

SOP, Lab Policies and Other Details

Publications

Publications
NA