Category
- Spectroscopy and Spectrometry ยป Mass Spectrometry
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Features:
Sub-ppm mass accuracy and 40,000 FSR full sensitivity resolution.
Extreme sensitivity across the entire mass ranges from very small molecules to intact proteins
High speed MS/MS capabilities with full U-HPLC compatibility.
Simultaneous analysis of major and trace sample components.
Isotopic fidelity for definitive molecular formulae determination.
Robust and easy to operate system
Certainty in identification in combination with Smart Formula 3DTM - unequivocal formula generation with superb mass accuracy.
Better than 0.8 ppm Mass accuracy
Working Principle:
Mass spectrometry is an analytical technique that can provide both qualitative (structure) and quantitative (molecular mass or concentration) information on analyte molecules after their conversion to ions. The molecules of interest are first introduced into the ionization source of the mass spectrometer, where they are first ionized to acquire positive or negative charges. The ions then travel through the mass analyzer and arrive at different parts of the detector according to their mass/charge (m/z) ratio. After the ions make contact with the detector, useable signals are generated and recorded by a computer system. The computer displays the signals graphically as a mass spectrum showing the relative abundance of the signals according to their m/z ratio.
Electrospray ionization mass spectrometry is a desorption ionization method. Desorption ionization methods can be performed on solid or liquid samples and allows for the sample to be nonvolatile or thermally unstable. This means that ionization of samples such as proteins, peptides, oligopeptides, and some inorganic molecules can be performed.
Some technical specifications:
The Bruker maXis Impact is a Hybrid Quadrupole/ Atmospheric pressure ionization orthogonal accelerated Time-of-flight mass spectrometer. The maXis impact is bench-top instrument which includes,
Apollo II Electrospray ion source
APCI II source.
Quadrupole MS/MS Filter
Orthogonal pulsed ion extraction and UHR Time of flight Mass analyzer.
Vacuum system.
Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges
External Users can come in-person or send your samples along with a letter from the Head / Guide on your College/Institute /Industry Original Letter Head for registration stating that the analysis is for research purpose to qualify for academic concession. The letter should be addressed to The Head, Dept. Of Chemistry, IIT, Bombay.
The analytical data / spectra are provided only for research / development purposes. These can not be used as certificates in legal disputes.
Samples, letter and payment should be sent in the same cover only. If Samples are received without covering letter/DD, the samples will be sent back to the user without any further intimation/notice. Separate Sample should be sent for different analysis.
Samples are not analysed till Payment is received.
Please send the samples in quantity as mentioned. Samples are not recovered, unless a special request is made.
Radioactive material should be clearly mentioned and handed over personally.
Unstable and explosive compounds are not accepted for analysis.
The payment should be made in advance by only Demand Draft (DD) in favour of: " The Registrar, IIT Bombay."
The DD along with sample and covering letter should be sent to :
The Head, Dept. Of Chemistry,
Mass Lab. Room No. 374, 2nd floor.
Indian Institute Of Technology, Bombay,
Powai, Mumbai-400076
The sample should be pure and dry and submit in a vial along with the sample code, molecular formula or molecular weight.
Mention the suitable solvent (preferably protonated solvent like MeOH, ACN or water.)
Applications
Proteins/Peptides:
In-depth qualitative analysis of complex samples
Intact mass measurement
Small Molecules:
Identification and characterization
Metabolite analysis:
Targeted and non -targeted approaches
Sample Details
NO