Fluorescence Spectrofluorometer

External users: registration to be carried out only through I-STEM portal
Additional information about sample and analysis details should be filled in the pdf form provided in the I-STEM portal under “DOWNLOAD CSRF”

Internal users (IITB): registration to be carried out only through DRONA portal
Additional information about sample and analysis details should be filled in the pdf form provided here.

PL-VIS-NIR_MSDS.pdf (188.05 KB)
Make
Horriba, Jobin Vyon
Model
Fluorolog -3 (Purchased in 2006)
Facility Status
Working
Date of Installation
Facility Management Division
Centre for Sophisticated Instruments and Facilities (CSIF)

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Category

  • Spectroscopy and Spectrometry » Optical Spectroscopy

Booking Details

Booking available for
Internal and External Both

Facility Management Team and Location

Facility In Charge
Head, CSIF
email: office.saif@iitb.ac.in
Facility Manager
Mrs.Sonali Prashant Duble
sonali@iitb.ac.in
Facility Operator
Sonali Duble
email ID-sonali@iitb.ac.in
Department
Centre for Sophisticated Instruments and Facilities (CSIF)
Lab Email ID
sonali@iitb.ac.in
Facility Location
413-B First Floor, SAIF IIT Bombay
Lab Phone No
022-2159 6869

Facility Features, Working Principle and Specifications

Features Working Principle

Specifications : Wavelength Range - 200 to 1500 nm
  Monochromator - 2 in series on excitation side and 1 - 1 each on visible and NIR side emission side
  Light Source - 450 W Xenon Lamp
  Scanning Speed - 150 nm/sec
  Wavelength Accuracy - 0.5 nm
  Integration time -1ms to 160 s
  Detectors: visible (200-800nm)-PMT and NIR (800-1500nm)-Liq.N2 cooled InGaAs
 

Accessories 

     1. Single Cell liquid Holder 
     2. Solid Sample Holder 
     3. Two Lasers 800nm and 980 nm are available for upconversion measuments 
      

Working Principle 

It is a rapid and sensitive method for the characterization of molecular components present in a sample. Fluorescence spectroscopy is a type of electromagnetic spectroscopy which analyzes fluorescent molecules. It exploits the phenomenon of electron excitation upon collision with high energy particles like photons and other excited electrons. In the process of transition of polyatomic fluorescent molecules (fluorophores) from their higher energy level (excited state) to a ground state by lowering their energy level, photons are emitted. Fluorophores play the most important role in fluorescence spectroscopy. Fluorophores are the components in molecules that impart fluorescence. Mostly, fluorophores are the molecules which contain aromatic rings such as tyrosine, tryptophan, fluorescein, etc. 

Sample Preparation, User Instructions and Precautionary Measures

User Instructions and Precautionary Measures

 Kindly download Analysis Request Form and Safety Data sheet and submit the hardcopy of filled sheet along with your sample
 

1. Provide solid samples in the powder form. Minimum required amount is approximately 40-50mg. 
2. Samples may be in liquid form. The required minimum amount is 2-3 ml. 
3. Samples can be submitted as thin film, Solid pellets, powder etc. 
4. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous. 
5. Samples will not be accepted unless MSDS is provided. 
6. Please send or submit Sample Details Form along with requisition letter and DD. -->
 

Fluorescence spectrofluorometer-system charges includes GST:

CategoryChargesGST@18%
IITB(TAs)50No GST
IITB( Students)100No GST
IITB-Monash Students100 
Academic Institutes200236
National Labs500590
SINE (Letter from SINE reqd)500590
Research Park (MSME) Letter from RP reqd500590

Research park (Big Industry partners) letter frm RP reqd)

and MSME not associated with RP (appropriate certificate required)

750885
Industries10001180


 

Charges for Analytical Services in Different Categories

Usage Charges

 

CategoryChargesGST@18%
IITB(TAs)50No GST
IITB( Students)100No GST
IITB-Monash Students100 
Academic Institutes200236
National Labs500590
SINE (Letter from SINE reqd)500590
Research Park (MSME) Letter from RP reqd500590

Research park (Big Industry partners) letter frm RP reqd)

and MSME not associated with RP (appropriate certificate required)

750885
Industries10001180

 

 

 


 

Applications

Environmental 
Industrial 
Medical diagnostics 
DNA sequencing 
Forensics 
Genetic analysis 
Biotechnology 
Qualitative and quantitative analysis 

Sample Details

SOP, Lab Policies and Other Details

Publications