Category
- Spectroscopy and Spectrometry ยป Optical Spectroscopy
Booking Details
Facility Management Team and Location
head.saif@iitb.ac.in
office.saif@iitb.ac.in
0091-22- 2576 7691/2
- Dr. Mayuri Gandhi, Research Scientist, SAIF
Email: mngandhi@iitb.ac.in - Dr. Gajender Saini, Senior Technical Officer, SAIF
Email: gsaini@iitb.ac.in
Email: naresh.ambati@iitb.ac.in
Email: nmagadi@iitb.ac.in
Email: bharati@iitb.ac.in
Facility Features, Working Principle and Specifications
Specifications : Wavelength Range - 200 to 1500 nm
Monochromator - 2 in series on excitation side and 1 - 1 each on visible and NIR side emission side
Light Source - 450 W Xenon Lamp
Scanning Speed - 150 nm/sec
Wavelength Accuracy - 0.5 nm
Integration time -1ms to 160 s
Detectors: visible (200-800nm)-PMT and NIR (800-1500nm)-Liq.N2 cooled InGaAs
Accessories
1. Single Cell liquid Holder
2. Solid Sample Holder
3. Two Lasers 800nm and 980 nm are available for upconversion measuments
Working Principle
It is a rapid and sensitive method for the characterization of molecular components present in a sample. Fluorescence spectroscopy is a type of electromagnetic spectroscopy which analyzes fluorescent molecules. It exploits the phenomenon of electron excitation upon collision with high energy particles like photons and other excited electrons. In the process of transition of polyatomic fluorescent molecules (fluorophores) from their higher energy level (excited state) to a ground state by lowering their energy level, photons are emitted. Fluorophores play the most important role in fluorescence spectroscopy. Fluorophores are the components in molecules that impart fluorescence. Mostly, fluorophores are the molecules which contain aromatic rings such as tyrosine, tryptophan, fluorescein, etc.
Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges
Kindly refer to the SAIF site for instruction for registration
Kindly download Analysis Request Form and Safety Data sheet and submit the hardcopy of filled sheet along with your sample
1. Provide solid samples in the powder form. Minimum required amount is approximately 40-50mg.
2. Samples may be in liquid form. The required minimum amount is 2-3 ml.
3. Samples can be submitted as thin film, Solid pellets, powder etc.
4. MS-DS (Material Safety Data Sheet) should be given along with samples to ensure that there sample being given. Samples should not be toxic or hazardous.
5. Samples will not be accepted unless MSDS is provided.
6. Please send or submit Sample Details Form along with requisition letter and DD. -->
Fluorescence spectrofluorometer-system charges includes GST:
|
Applications
Environmental
Industrial
Medical diagnostics
DNA sequencing
Forensics
Genetic analysis
Biotechnology
Qualitative and quantitative analysis