Cryo high resolution transmission electron microscope facility
Make
JEOL, JEM 2100
Model
HRTEM
Facility Status
Working
Facility Management Division
Institute Central Research Facilities (ICRF)

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Category

  • Microscopy and Imaging » Electron Microscopy

Booking Details

Booking available for
Internal and External Both
Available Equipment/ Mode of use
Room temperature imaging of dry samples Cryo mode-Imaging of vitrified sample
EDS-Elemental mapping
EELS-Electron energy loss spectroscopy STEM-Brightfield and darkfield images Electron diffraction for crystallography studies

Facility Management Team and Location

Faculty In Charge
Prof. J Bellare jb@iitb.ac.in +91 (22) 2576 7207
Co-convenors
Prof. J Bellare Prof. D Panda Prof. I Samajdar Prof. S S Major Prof. A Mehra Prof. R M Thaokar
Department
Chemical Engineering
Lab Email ID
bioafm@iitb.ac.in
Facility Location
Room No.110, Ground floor, Silicate Lab, Chemical Engg Dept. Powai, Mumbai - 400 076.
Lab Phone No
+91 022 25764266

Facility Features, Working Principle and Specifications

Features Working Principle

Working Principle

This JEM 2100 ultra HRTEM 200kVwas installed at the IIT Bombay in June 2010 as part of an DST-IRPHA project with partial support from the Institute. This HRTEM facility is equipped with a few useful attachments such as a scanning image observation device that incorporates provisions for obtaing STEM images, an EELS, an EDS, and most importantly, a Cryo mode facility with cryo specimen holder, cryo transfer unit, dry pumping station, and cryo sample preparation equipment like Gatan Cryo Plunger and FEI Vitrobot, and cryo specimen holder. This facility is supported by IRCC as a Central Facility of the Institute. This facility is open for all IIT Bombay internal users to meet their research necessities. Due to the sophisticated nature of the equipment and elaborate set up, this instrument does see periods of downtime.

Body Specification

Transmission Electron Microscope Configuration:

Resolution:

  • Point resolution: 0.194 nm
  • Lattice resolution: 0.14 nm
  • Actual performance may vary for typical user samples.

Optical System:

  • Focal Length: 1.9 mm
  • Max. Eucentric Tilt: ±80°
  • Magnification: 50x to 1,500,000x

Electron Source:

  • LaB6 emitter
  • Accelerating Voltage: 80, 100, 120, 160, 200 kV

Vacuum System:

  • Fully interlocked
  • Differentially pumped column
  • Ultra High Vacuum for contamination-free observation (2 × 10-5 Pa)

Specimen Stage - Compustage:

  • Fully computer controlled, Eucentric side entry, high stability
  • High tilt and large field of view
  • Choice of variety of Specimen Holders (Cryo, Multiple, Rotation Holders, etc.)
  • X, Y movement: 2 mm; specimen size: 3 mm
  • Specimen position store and recall including magnification and illumination settings
  • Specimen exchange without switching off High Tension and Emitter

Imaging:

  • High contrast
  • Automated contrast enhancement function
  • Focusing aid, adjustable wobbler for all magnification and directions
  • Rotation-free magnification and diffraction series
  • Magnification aberration corrected lens series
  • Embedded CCD Camera - Gatan
  • Low dose software
  • Image acquisition software with wide choice for image manipulation and analysis

Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures

Charges for Analytical Services in Different Categories

Applications

Nanotechnology, Biology and Life sciences, Material science, Pharmaceutical analysis, Semiconductors.

Sample Details

SOP, Lab Policies and Other Details

Publications

List of publications
1. In Vivo Analysis of Biodegradable Liposome Gold Nanoparticles as Efficient Agents for Photothermal Therapy of Cancer AK Rengan, AB Bukhari, A Pradhan, R Malhotra, R Banerjee, Nano letters 15 (2), 842-848
2. Mayur K Temgire, Akkihebbal K. Suresh,  Shantaram G. Kane,and Jayesh       Bellare Establishing The Interfacial Nano-structure And Elemental Composition of Homeopathic Medicines Based on Inorganic Salts: A Scientific Approach - September 2015 10.1016/j.homp.2015.09.006
3. Formation of nanoparticles and nanocrystals of mercury by α-lactalbumin Chebrola Pulla Rao. Atul Gajanan Thawari . Vijaya Kumar hinge · Mayur Temgire RSC Advances 09/2014; DOI:10.1039/C4RA07156E
4. Chain length dependence of polyol synthesis of zinc ferrite nanoparticles: Why is diethylene glycol so different? Supriya N Rishikeshi· Satyawati S Joshi · Mayur K Temgire · Jayesh R Bellare, Dalton Transactions 02/2013; 42(15). DOI:10.1039/c2dt32026f 
5. Redox Decomposition of Silver Citrate Complex in Nanoscale Confinement: An Unusual Mechanism of Formation and Growth of Silver Nanoparticles  Sabyasachi Patra†, Ashok K. Pandey†, Debasis Sen‡, Shobha V. Ramagiri§, Jayesh R. Bellare§, S. Mazumder‡, and A. Goswami*†  langmuir, 2014, 30 (9), pp 2460–2469
6. Enhancing cubosome functionality by coating with a single layer of poly-ε-lysine Deshpande S1, Venugopal E, Ramagiri SV, Bellare JR, Kumaraswamy G, Singh N.Volume 6, Issue 19, 8 October 2014, Pages 17126-17133 ACS Appl. Mater. Interfaces 
7. Aravind Kumar Rengan, Amirali B Bukhari, Arpan Pradhan, Renu Malhotra, Rinti Banerjee, Rohit Srivastava, Abhijit De, (2015) Invivo analysis of biodegradable liposomes gold nanoparticles as efficient agents for photothermal therapy of cancer. Nanoletters, 12, 19.
8. Supriya N Rishikeshi, Satyawati S Joshi, Mayur K Temgire, Jayesh R Bellare,  Chain length dependence of polyol synthesis of zinc ferrite nanoparticles: why is diethylene glycol so different? Dalton Trans 2013 Apr 19;42(15):5430-8.
9. Chhabra, H., Gupta, P., Verma, P.J., Jadhav, S., Bellare, J.R.;Gelatin-PMVE/MA composite scaffold promotes expansion of embryonic stem cells; (2014) Materials Science and Engineering C, 37 (1), pp. 184-194.
10. Jaiswal, A.K., Dhumal, R.V., Ghosh, S., Chaudhari, P., Nemani, H., Soni, V.P., Vanage, G.R., Bellare, J.R.; Bone healing evaluation of nanofibrous composite scaffolds in rat calvarial defects: A comparative study; (2013) Journal of Biomedical Nanotechnology, 9 (12), pp. 2073-2085.
11. Jaiswal, A.K., Dhumal, R.V., Bellare, J.R., Vanage, G.R.; In vivo biocompatibility evaluation of electrospun composite scaffolds by subcutaneous implantation in rat; (2013) Drug Delivery and Translational Research, 3 (6), pp. 504-517.
12. Singh, R., Wagh, P., Wadhwani, S., Gaidhani, S., Kumbhar, A., Bellare, J., Chopade, B.A.; Synthesis, optimization, and characterization of silver nanoparticles from Acinetobacter calcoaceticus and their enhanced antibacterial activity when combined with antibiotics; (2013) International Journal of Nanomedicine, 8, pp. 4277-4290.
13. Sagar, N., Pandey, A.K., Gurbani, D., Khan, K., Singh, D., Chaudhari, B.P., Soni, V.P., Chattopadhyay, N., Dhawan, A., Bellare, J.R.; In-Vivo Efficacy of Compliant 3D Nano-Composite in Critical-Size Bone Defect Repair: A Six Month Preclinical Study in Rabbit; (2013) PLoS ONE, 8 (10), art. no. e77578, 
14. Kanitkar, M., Jaiswal, A., Deshpande, R., Bellare, J., Kale, V.P.; Enhanced Growth of Endothelial Precursor Cells on PCG-Matrix Facilitates Accelerated, Fibrosis-Free, Wound Healing: A Diabetic Mouse Model; (2013) PLoS ONE, 8 (7), art. no. e69960. </li>
2019-2020
1. Effects of Ethanol Addition on the Size Distribution of Liposome Suspensions in Water, Ankush Pal, P. Sunthar, D. V. Khakhar*, Ind. Eng. Chem. Res. 2019, 58, 18, 7511–7519
2. Efficient separation of biological macromolecular proteins by polyethersulfone hollow fiber ultrafiltration membranes modified with Fe3O4 nanoparticles …, A Modi, J Bellare - International journal of biological macromolecules, 2019 - Elsevier
3. The consequence of silicon additive in isothermal decomposition of hydrides LiH, NaH, CaH2 and TiH2 R Kalamkar, V Yakkundi, A Gangal - … Journal of Hydrogen Energy, 2020
4. Synthesis and properties of amino and thiol functionalized graphene oxide, HP Manwatkar, SD Gedam, CS Bhaskar… - Materials Today …, 2020 - Elsevier
5. Impact of thermal annealing inducing oxidation process on the crystalline powder of In 2 S 3, A Timoumi, W Zayoud, A Sharma, M Kraini… - Journal of Materials …, 2020 - Springer
6. Unique Structure-Induced Magnetic and Electrochemical Activity in Nanostructured Transition Metal Tellurates Co1 – xNixTeO4 (x = 0, 0.5, and 1)copyright © 2020 American Chemical Society
7. Amoxicillin removal using polyethersulfone hollow fiber membranes blended with ZIF-L nanoflakes and cGO nanosheets: Improved flux and fouling-resistance, A Modi, J Bellare - Journal of Environmental Chemical Engineering, 2020 - Elsevier
8. La/Ce mixed metal oxide supported MWCNTs as a heterogeneous catalytic system for the synthesis of chromeno pyran derivatives and assessment of green …, RA Rather, S Siddiqui, WA Khan, ZN Siddiqui - Molecular Catalysis, 2020 - Elsevier
9. Compositional Control as the Key for Achieving Highly Efficient OER Electrocatalysis with Cobalt Phosphates Decorated Nanocarbon Florets, J Saha, S Verma, R Ball, C Subramaniam… - Small, 2020 - Wiley Online Library
10. Hybrid silver–gold nanoparticles suppress drug resistant polymicrobial biofilm formation and intracellular infection, E Bhatia, R Banerjee - Journal of Materials Chemistry B, 2020 - pubs.rsc.org
11. Development of oxidation resistant and mechanically robust carbon nanotube reinforced ceramic composites, V Verma, SC Galaveen, L Gurnani… - Ceramics …, 2020 - Elsevier
12. Zeolitic imidazolate framework-67/carboxylated graphene oxide nanosheets incorporated polyethersulfone hollow fiber membranes for removal of toxic heavy …, A Modi, J Bellare - Separation and Purification Technology, 2020 - Elsevier
13. Enhanced wettability and photocatalytic activity of seed layer assisted one dimensional ZnO nanorods synthesized by hydrothermal method, D Upadhaya, DD Purkayastha - Ceramics International, 2020 - Elsevier
14. Di-tert-butylphosphate Derived Thermolabile Calcium Organophosphates: Precursors for Ca(H2PO4)2, Ca(HPO4), α-/β-Ca(PO3)2, and Nanocrystalline Ca10(PO4)6 …, S Verma, R Murugavel - Inorganic Chemistry, 2020 - ACS Publications 
For the Year 2021
1. Probing Kinetics and Mechanism of Formation of Mixed Metallic Nanoparticles in a Polymer Membrane by Galvanic Replacement between Two Immiscible Metals ... NG Gaidhani, S Patra, HS Chandwadkar, D Sen... - Langmuir, 2021 - ACS Publication. ...
2. Influence of carbon nanotube type and novel modification on dispersion, melt‐rheology and electrical conductivity of polypropylene/carbon nanotube composites J Banerjee, S Kummara, AS Panwar... - Polymer ..., 2021 - Wiley Online Library
3. Composition uniformity and large degree of strain relaxation in MBE-grown thick GeSn epitaxial layers, containing 16% Sn J Rathore, A Nanwani, S Mukherjee... - Journal of Physics D ..., 2021 - iopscience.iop.org
4. Hydrothermal-assisted synthesis and stability of multifunctional MXene nanobipyramids: structural, chemical, and optical evolution B Singh, R Bahadur, S Neekhra, M Gandhi... - ... Applied Materials & ..., 2021 - ACS Publications
5. Influence of carbon nanotube type and novel modification on dispersion, melt‐rheology and electrical conductivity of polypropylene/carbon nanotube composites J Banerjee, S Kummara, AS Panwar... - Polymer ..., 2021 - Wiley Online Library
6. Interaction of Aromatic Amino Acid-Functionalized Gold Nanoparticles with Lipid Bilayers: Insight into the Emergence of Novel Lipid Corona Formation A Maity, SK De, A Chakraborty - The Journal of Physical Chemistry ..., 2021 - ACS Publications
7. Ultra-fast, chemical-free, mass production of high quality exfoliated graphene A Islam, B Mukherjee, KK Pandey, AK Keshri - ACS nano, 2021 - ACS Publications
8. Multifunctional hybrid soret nanoarchitectures for mobile phone-based picomolar Cu2+ ion sensing and dye degradation applications S Bhaskar, P Jha, C Subramaniam... - Physica E: Low ..., 2021 - Elsevier
9. Polyphenol stabilized copper nanoparticle formulations for rapid disinfection of bacteria and virus on diverse surfaces K Sadani, P Nag, L Pisharody, XY Thian, G Bajaj... - ..., 2021 - iopscience.iop.org
10. Bicomponent Coassembled Hydrogel as a Template for Selective Enzymatic Generation of DOPAS Biswas, T Ghosh, DKK Kori, AK Das - Langmuir, 2021 - ACS Publications
11. Improved non-enzymatic H2O2 sensors using highly electroactive cobalt hexacyanoferrate nanostructures prepared through EDTA chelation route R Banavath, R Srivastava, P Bhargava - Materials Chemistry and Physics, 2021 - Elsevier
12. Enhanced antibacterial activity of decahedral silver nanoparticles S Bharti, S Mukherji, S Mukherji - Journal of Nanoparticle Research, 2021 - Springer
13. Non-Enzymatic H2O2 Sensor Using Liquid Phase High-Pressure Exfoliated Graphene R Banavath, SS Nemala, R Srivastava... - Journal of The ..., 2021 - iopscience.iop.org
14. Spontaneous Ion Migration via Mechanochemical Ultrasonication in Mixed Halide Perovskite Phase Formation:
Experimental and Theoretical Insights M Roy, Vikram, Bhawna, U Dedhia... - The Journal of ..., 2021 - ACS Publications
15. Zeolitic imidazolate framework-8 nanoparticles coated composite hollow fiber membranes for CO2/CH4 separation K Sainath, P Kumari, J Bellare - Journal of Environmental Chemical ..., 2021 - Elsevier
16. Non-stoichiometry induced exsolution of metal oxide nanoparticles via formation of wavy surfaces and their enhanced electrocatalytic activity: case of misfit calcium ... KS Roy, C Subramaniam... - ACS Applied Materials & ..., 2021 - ACS Publications
17. Ion-Induced Bending with Applications for High-Resolution Electron Imaging of Nanometer-Sized Samples S Zhang, V Garg, G Gervinskas... - ACS Applied Nano ..., 2021 - ACS Publications
18. Synthesis, Characterization, and Hydrogen Gas Sensing of ZnO/g-C3N4 Nanocomposite A Ibrahim, UB Memon, SP Duttagupta... - Engineering ..., 2021 - mdpi.com
19. Underlying mechanisms for the modulation of self-assembly and the intrinsic fluorescent properties of amino acid- functionalized gold nanoparticles SK De, A Maity, A Chakraborty - Langmuir, 2021 - ACS Publications
20. [HTML] Novel combination of bioactive agents in bilayered dermal patches provides superior wound healing MM Pillai, H Dandia, R Checker, S Rokade... - ... , Biology and Medicine, 2022 - Elsevier... Murine
21. Probing Kinetics and Mechanism of Formation of Mixed Metallic Nanoparticles in a Polymer Membrane by Galvanic Replacement between Two Immiscible Metals ... ..., D Sen, C Majumder, SV Ramagiri, JR Bellare - Langmuir, 2021 - ACS Publications
22. Concomitant Effect of Quercetin-and Magnesium-Doped Calcium Silicate on the Osteogenic and Antibacterial Activity of Scaffolds for Bone Regeneration AM Preethi, JR Bellare - Antibiotics, 2021 - mdpi.com
23. “Viscotaxis”-directed migration of mesenchymal stem cells in response to loss modulus gradient..., V Kumar, D Shah, S Beri, S Das, J Bellare... - Acta biomaterialia, 2021 - Elsevier
24. Matrix dependent spatial distributions of in situ formed rhodium nanostructures in ion-exchange membranes..., AK Pandey, SV Ramagiri, JR Bellare - Materials Today Chemistry, 2021 - Elsevier
25. pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages KB Cotta, S Mehra... - Beilstein journal of ..., 2021 - beilstein-journals.org
26. A large area flexible p-type transparent conducting CuS ultrathin films generated at liquid-liquid interface SS Pathak, LS Panchakarla - Applied Materials Today, 2021 - Elsevier