Cryo high resolution transmission electron microscope facility
Cryo high resolution transmission electron microscope facility
Make
JEOL, JEM 2100
Model
HRTEM
Facility Status
Working
Facility Management Division
Institute Central Research Facilities (ICRF)

Category

  • Microscopy and Imaging » Electron Microscopy

Booking Details

Booking available for
Internal and External Both
Available Mode for Use
Room temperature imaging of dry samples Cryo mode-Imaging of vitrified sample
EDS-Elemental mapping
EELS-Electron energy loss spectroscopy STEM-Brightfield and darkfield images Electron diffraction for crystallography studies

Facility Management Team and Location

Faculty In Charge
Prof. J Bellare jb@iitb.ac.in +91 (22) 2576 7207
Co-convenors
Prof. J Bellare Prof. D Panda Prof. I Samajdar Prof. S S Major Prof. A Mehra Prof. R M Thaokar
Department
Chemical Engineering
LAB Email ID
bioafm@iitb.ac.in
Facility Location
Room No.110, Ground floor, Silicate Lab, Chemical Engg Dept. Powai, Mumbai - 400 076.
Lab Phone No
+91 022 25764266

Facility Features, Working Principle and Specifications

Features Working Principle

Working Principle

This JEM 2100 ultra HRTEM 200kVwas installed at the IIT Bombay in June 2010 as part of an DST-IRPHA project with partial support from the Institute. This HRTEM facility is equipped with a few useful attachments such as a scanning image observation device that incorporates provisions for obtaing STEM images, an EELS, an EDS, and most importantly, a Cryo mode facility with cryo specimen holder, cryo transfer unit, dry pumping station, and cryo sample preparation equipment like Gatan Cryo Plunger and FEI Vitrobot, and cryo specimen holder. This facility is supported by IRCC as a Central Facility of the Institute. This facility is open for all IIT Bombay internal users to meet their research necessities. Due to the sophisticated nature of the equipment and elaborate set up, this instrument does see periods of downtime.

Body Specification

Transmission Electron Microscope Configuration:

Resolution:

  • Point resolution: 0.194 nm
  • Lattice resolution: 0.14 nm
  • Actual performance may vary for typical user samples.

Optical System:

  • Focal Length: 1.9 mm
  • Max. Eucentric Tilt: ±80°
  • Magnification: 50x to 1,500,000x

Electron Source:

  • LaB6 emitter
  • Accelerating Voltage: 80, 100, 120, 160, 200 kV

Vacuum System:

  • Fully interlocked
  • Differentially pumped column
  • Ultra High Vacuum for contamination-free observation (2 × 10-5 Pa)

Specimen Stage - Compustage:

  • Fully computer controlled, Eucentric side entry, high stability
  • High tilt and large field of view
  • Choice of variety of Specimen Holders (Cryo, Multiple, Rotation Holders, etc.)
  • X, Y movement: 2 mm; specimen size: 3 mm
  • Specimen position store and recall including magnification and illumination settings
  • Specimen exchange without switching off High Tension and Emitter

Imaging:

  • High contrast
  • Automated contrast enhancement function
  • Focusing aid, adjustable wobbler for all magnification and directions
  • Rotation-free magnification and diffraction series
  • Magnification aberration corrected lens series
  • Embedded CCD Camera - Gatan
  • Low dose software
  • Image acquisition software with wide choice for image manipulation and analysis

Instructions for Registration, Sample Preparation, User Instructions, Precautionary Measures and Charges

Applications

Nanotechnology, Biology and Life sciences, Material science, Pharmaceutical analysis, Semiconductors.

Sample Details

SOP, Lab Policies and Other Details

Publications

Publications
Publications