.
Category
- Fabrication and Processing » Microfabrication
Booking Details
Facility Management Team and Location
Facility Features, Working Principle and Specifications
Facility Description
The CELLINK 3D Bioprinter is an advanced system for fabricating tissue-like structures using bioinks with living cells, biomaterials, and growth factors. With high-precision extrusion, multi-material printing, and temperature control
The Cellink BIO X6 3D bioprinter operates on the principle of additive manufacturing, where biological components, biochemicals, and living cells are precisely deposited in a layer-by-layer fashion to fabricate complex three-dimensional structures. This process is guided by a digital design file, ensuring accurate spatial control over the placement of functional constituents
- Theoretical resolution XY: - 1μm
- Theoretical layer resolution Z:- 1μm
- Software Resolution XYZ:- 10μm–
- Pressure range (internal pump) :- 0–200 kPa
- Pressure range (external air supply) :- 0-700 kPa
- Output Pressure Offset:-+/- 5 kPa
- No. of printhead slots - 6
- Photocuring sources (built-in):- 365 nm, 405 nm, 475 nm, 520 nm
- Printbed temperature range :- 4-65°C
- Printhead temperature range :- 4-250°C
- Pneumatic printhead temperature range:- 30–65°C
- Filter class, chamber airflow :- HEPA 14
- UV sterilization :- UV-C (275 nm) 2W output
- Dimensions:- 800 mm (L) × 400 mm (W) × 500 mm (H)
- Weight :- 47.4 kg (104.5 lbs)
- Supported file formats:- G-code, stl, amf, 3mf, obj.
Instructions for Registration, Sample Preparation, User Instructions and Precautionary Measures
- User should check the availability of facility.
- Prepare your bioink with optimized viscosity, biocompatibility, and printability to ensure precise and stable 3D bioprinting
- Select the appropriate nozzle and cartridge based on the bioink viscosity to ensure smooth extrusion and precise 3D bioprinting.
- beside this user should strictly follow the SOPs while the usage of facility
1. Bioink Preparation
Selection: Choose bioink based on biocompatibility, viscosity, and crosslinking needs.
Mixing: Thaw, pre-warm, and homogenize bioink.
Cell Encapsulation: Harvest, centrifuge, and mix cells into bioink under sterile conditions.
2. Printing Surface Preparation
Surface Selection: Use multiwell plates, petri dishes, or scaffolds.
Coating (if needed): Apply collagen or fibronectin for better adhesion.
Sterilization: Use UV light, autoclaving, or ethanol.
3. Loading Bioink into Printer
Cartridge Preparation: Load bioink, remove air bubbles, and seal.
Nozzle Selection: Choose an appropriate diameter for resolution and viscosity.
Temperature Control: Adjust printhead temperature as required.
4. Pre-Printing Checks
Calibration: Align nozzle and perform test prints.
Crosslinker Preparation: Prepare appropriate agents for stabilization.
5. Post-Printing Care
Stabilization: Apply crosslinking agents promptly.
Incubation: Transfer constructs to an incubator (e.g., 37°C, 5% CO₂).
Cleaning: Sterilize nozzles, cartridges, and printbed after use.
Reference:-Mandrycky et al. (2016). 3D Bioprinting for Engineering Complex Tissues. Biotechnology Advances, 34(4), 422-434.
Charges for Analytical Services in Different Categories
Charges for Internal Users (Excluding of GST):-
| Description | Charge (INR) |
| IITB TAs for the instrument | 125/- |
| Other IITB Users | 250/ |
| Start up (SINE incubation) | 500/-* |
| Monash-IITB | 250/-* |
Charges for External Users* (Excluding of GST):
| Description | Charge (INR) |
| Academic | 250/-* |
| National R & D | 500/-* |
| Industry/Non Govt. Agencies | 1250/-* |
| Other Countries (Academic) | 1250/- |
| Other Countries (Industry) | 2500/ |
Applications
- Biofabrication of Functional Biomaterials
- Disease Modeling & Drug Testing
- Tissue Engineering & Regenerative Medicine
- Scaffold Preparation.
- Research and Education
Sample Details
02-100mm in length and width, with a thickness of 1-5mm
10-100 micrometers (µm)
Sterile
NA
SOP, Lab Policies and Other Details
Publications
NA